摘要
通过多采酶链反应（ＰＣＲ）及限制酶分析，确定白血病克隆ＴｃＲγ重排方式。１８例发生ＴｃＲγ重排的ＡＬＬ病人中，４例初诊与复发时重排方式发生变化。ＴｃＲγ重排变化的发生不限于免疫表型或某一年龄的病人。病程中ＴｃＲγ基固重排的变化反映了白血病细胞仍可不断演化，是白血病克隆演化的基因证据。探讨了这一研究的临床意义。
In this article,we studied the clonal evolution of leukemia by the rearrangementof T-cell receptor γ cliain (TcRγ) genes. The rearrangement types of TcRγ genes of a leukemicclone were identified by poly,merase chain reaction(PCR) and restriction analysis. In 18 cases ofALLs with rearranged TcRγ genes,the changes of rearrangement types from presentation torelapse were confirmed in 4 cases. The occurrence of changes of rearrangement types was notrestricted to any particular immunophenotype or age group. The changes during disease coursereflected the fact that leukemic cell populatiOn can differentiate continually after transformation,and provided a genic evidence for the clonal evolution of leukemia. We tried to inquire into thecIinical implications of this study.
出处
《白血病》
1996年第2期83-87,共5页
关键词
克隆演化
白血病
T细胞受体
聚合酶链反应
Clonal evolution Leukemia T-cell receptor gene rearrangement Polymerasechain reaction
