摘要
Objective: This study aimed to establish chemiluminescent immunoassay (CLIA) for quantitative determination of theophylline levels in human serum. Methods: To measure the concentration of theophylline (n=122) and evaluate the assay.Results: The linear range of the CLIA method was 0.51～40 mg/L (Y=1.02X+0.44, r=0.995). The intra and inter CV (coefficient variance) of CLIA were 3.20% and 3.57%, respectively. The average recovery rate was 102.3%. This method was free from interference by brilirubin (＜200 μmol/L), hemoglobin (＜10 g/L), and triglycerides (＜15 mmol/L). Conclusion: This method is simple, convenient and precise for clinical pharmacokinetics study oftheophylline.
Objective： This study aimed to establish chemiluminescent immunoassay （CLIA） for quantitative determination of theophylline levels in human serum. Methods： To measure the concentration of theophylline （n=122） and evaluate the assay. Results： The linear range of the CLIA method was 0.51-40 mg/L （Y=1.02X＋0.44, r=0.995）. The intra and inter CV （coefficient variance） of CLIA were 3.20% and 3.57%, respectively. The average recovery rate was 102.3%. This method was free from interference by brilirubin （〈200μmol/L）, hemoglobin （〈10g/L）, and triglycerides （〈15 mmol/L）. Conclusion： This method is simple, convenient and precise for clinical pharmacokinetics study of theophylline.
