摘要
目的建立一种小鼠心脏骤停模型,为心肺复苏的实验研究奠定基础.方法选用健康小鼠15只,体重30～50g,雌雄不限,麻醉后插入导管至左颈总动脉测压,实验中全程监测心电图.应用经食管心脏起搏的方法对小鼠诱发室颤或无脉性电活动并维持4 min,然后开始常规心肺复苏.结果经食管心脏快速起搏可以导致血压迅速下降,停止心脏起搏刺激后,15只小鼠中有14只心电图表现为无脉性电活动,1只表现为一条直线,未诱出持续性室颤.全部小鼠均复苏成功.结论经食管心脏起搏诱发小鼠心脏骤停的动物模型具有操作简单、重复性好和模型稳定的特点,能够满足心肺复苏基础研究的需要.
Objective To establish cardiac arrest models of the mice that would lay a foundation for the experimental studies of cardiopulmonary resuscitation.Methods 15 mice without distinction of sex （body weight 30 ～ 50 g） were anesthetized and a catheter was inserted into the left carotid artery for the measurement of aortic pressure. ECG was monitored continuously during the experiment. Ventricular fibrillation or pulseless electrical activity was induced and maintained by transoesophageal cardiac pacing. Conventional cardiopulmonary resuscitation began 4 min later. Results Transoesophageal fast cardiac pacing caused rapid drop of blood pressure in all the 15 animals. ECG showed pulseless electrical activity in 14 of 15 animals, asystole was in the remaining one animal and no sustained ventricular fibrillation was induced after the cardiac pacing was terminated. All the animals were successfully resuscitated with cardiopulmonary resuscitation 4 min after cardiac arrest. Conclusion The cardiac arrest model of the mice induced by transoesophageal cardiac pacing has the satisfactory operability, repetition and stability, which can satisfy fundamental study on cardiopulmonary resuscitation.
出处
《中国急救医学》
CAS
CSCD
北大核心
2005年第12期897-898,共2页
Chinese Journal of Critical Care Medicine
基金
广西自然科学基金资助项目(桂科自0135038)
关键词
心脏骤停
心肺复苏
模型
动物
大鼠
Cardiac arrest
Cardiopulmonary resuscitation
Model
Animal
Rat
