摘要
目的观察当归注射液及其分离组分的抗脑缺血作用。方法分别给予脑缺血大鼠(结扎双侧颈动脉制得)当归注射液及其分离组分Angi-S和Angi-Y,2周后测定大鼠脑超氧化物歧化酶(SOD)活性和一氧化氮(NO)含量,血清NO和丙二醛(MDA)的含量以及大脑皮质固缩神经细胞的数量。结果当归分离组分Angi-S和Angi-Y能使脑缺血大鼠脑SOD的活性显著升高(均P<0.05)、脑NO的含量显著下降(均P<0.05);Angi-S能使大鼠血清内MDA的含量下降(P<0.05);当归注射液、Angi-S和Angi-Y均可明显减少脑缺血大鼠大脑皮质固缩神经细胞的数量(均P<0.01);当归注射液和Angi-S均可明显减少脑缺血大鼠大脑海马h1段固缩神经细胞的数量(均P<0.05)。结论当归注射液及其分离组分Angi-S和Angi-Y均有抗脑缺血作用。
Objective To study the protective effects of the Chinese Angelica injection and its 2 components Angi-S and Angi-Y on rats with cerebral ischemia. Methods 72 SD rats served as the subjects of the study. The model of cerebral ischemia was set up by ligating bilateral common carotid arteries under urethan anesthesia. The 2 components Angi-S and Angi-Y were separated from the Chinese Angelica injection with silica gel column chromatography. The rats were randomly divided into several groups including the model group and trial group 1 to 3. Rats of the trial group 1 were given each an IP injection of 6.24 mL · kg^-1, 12.48 mL · kg^-1 and 18.72 mL · kg^-1 of Chinese Angelica injection ,q,d,. respectively. Rats of the trial group 2 were given each an IP injection of 6.24 mL · kg^-1 , 12.48 mL · kg^-1 and 18.72 mL · kg^-1 of Angi-S, q. d. , respectively. Rats of the trial group 3 were given each an IP injection of 6.24 mL · kg^-1 , 12.48 mL · kg^-1 and 18.72 mL · kg^-1of Angi-Y, q. d. , respectively. Rats of the model group were given equivalent volumes of normal satine in the same manner. The course of treatment in rats of all groups lasted 2 weeks. The animals were than sacrificed and serum and brain samples collected for the determination of nitric oxide（NO） and MDA in the sera, NO and SOD in the brain homogenates. Results Angi-S （in medium, high doses） and Angi-Y（ in low, medium and high doses）were shown to increase the SOD activities in the brain homogenate as compared with those of the model group （ P 〈 0.05 ）. Angi-S and Angi-Y , both in medium and high doses, were shown to decrease the content of NO in the brain homogenates as compared with that of the model group （ P 〈 0.01 ）. Angi-S in medium and high doses was shown to decrease the content of serum MDA as compared with the model group （ P 〈 O. 05 ）. The Chinese Angelica injection in medium and high doses , Angi-S and Angi-Y in low , medium and high doses were shown to decrease the number of pyknotic nerve cells in the cerebral cortex as compared with the model group （ P 〈 0. 05 or P 〈 0.01 ）. The Chinese Angelica injection in medium and high doses , Angi-S in medium and high doses were shown to decrease the number of pyknotic nerve cells in the hl area of the hippocampus as compared with the model group （P 〈 0. 05）. Conclusion The Chinese Angelica injection as well as its active components Angi-S and Angi-Y were shown to have protective effects on the rats with cerebral ischemia.
出处
《医药导报》
CAS
2005年第12期1092-1095,共4页
Herald of Medicine
基金
湖北省科技厅重点基金资助项目(基金编号:2002AA302B16)
