摘要
采用合成多肽抗原建立了检测抗戊型肝炎病毒(HEV)-IgM 的酶联免疫吸附试验(ELISA)方法,成本低、操作简便、快速、重复性好、特异性强。与抗甲型肝炎病毒(HAV)、乙型肝炎病毒(HBV)、丙型肝炎病毒(HCV)的 IgM 抗体无交叉反应,排除了 RF 的干扰。且可被β-巯基乙醇抑制而不起反应。检测健康献血员血清,抗 HEV-IgM 均为阴性;检测抗 HEV-IgG 阳性的戊型肝炎病人血清,抗 HEV-IgM 的阳性检出率为44%。检测抗 HAV-IgM 阴性的血清标本,抗 HEV-IgM 的阳性检出率为12%。此外,对45例疑似戊型肝炎病人急性期与恢复期血清的检测结果显示,前者的检出率(71%)高于后者(24%)。提示戊型肝炎病毒抗体的检出模式有三种,①IgG、IgM 抗体均阳性;②IgG 阳性、IgM 阴性;③IgG 阴性、IgM 阳性。表明抗 HEV-IgM 在发病早期出现,多在恢复期消失。抗 HEV-IgM 的检测对于戊型肝炎的早期诊断治疗及病程观察有重要意义,值得推广应用。
In this study,an indirect anti-HEV IgM ELISA was established by using synthetic peptides.It is cheap,easy,rapid,reproducible,and specific.There were neither cross-reaction with IgM for hepatitis A,B and C, nor interference by rheumatoid-factor.The specific HEV-IgM can he inhibited by β-ME.The HEV-IgM positive rate of hepatitis E patients(anti-HEV IgG positive)was 44%(45/103).The results for sera of 45 healthy donors were all HEV-IgM negative.6 of 50 acute hepatitis patients whose sera were negative for anti-HAV IgM were positive for anti- HEV IgM,and finally clinically diagnosed as hepatitis E.In addition,anti-HEV IgM was detected in paired sera of 45 patients.The positive rate was 71% in acute sera and 24% in convalescent sera.The results also pointed out the de- tection model of anti-HEV IgM:(1)IgG+,IgM+;(2)IgG-,IgM+;(3)IgG+,IgM-.The data indicated that anti-HEV IgM is a useful and reliable marker for the diagnosis and treatment of hepatitis E.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
1996年第2期146-148,共3页
Chinese Journal of Experimental and Clinical Virology
关键词
戊型肝炎病毒
病毒抗体
IGM
ELISA
Enzyme-linked immunosorbent assay
Hepatitis E virus
Antibody
viral
IgM/analysis
