摘要
目的 探讨弥漫性脑损伤后神经细胞凋亡及脑组织水肿情况及神经生长因子对此的干预作用.方法 采用Marmarou脑损伤模型.将Spraque-Dawley鼠40只随机分成脑损伤组,神经生长因子治疗组和生理盐水对照组.治疗组分别于致伤后10 min和60 min腹腔内注射神经生长因子(30 mg/kg),对照组注射等量生理盐水,于伤后24h、48 h、72 h和96 h断头取脑,采用原位末端标记法检测凋亡细胞,对比各时段脑水肿和神经细胞的凋亡情况.结果 神经细胞从伤后24 h出现细胞凋亡,48～72 h达高峰,治疗组与损伤组在24 h,48 h,72 h和96 h时脑组织含水量有显著性差异(P＜0.05),对照组与损伤组含水量无显著性差异(P＞0.05).结论 神经生长因子对脑损伤性脑水肿及神经细胞凋亡有治疗作用.
Objective To study the therapeutic effects of nerve growth factor （NGF） on neuron apoptosis and brain edema after diffuse brain injury. Methods The animal model was established by Marmarou equipment. 40 of Spraque - Dawley rats were divided into three groups at random： injury group, NGF group and control group. NGF was injected （30 mg/kg） via peritoneum two times at the l0 and 60 min after brain injury in NGF group rats, At the same time saline was injected in control group, At the times of 24, 48, 72 and 96 hours, the rats were sacrificed by decapitation. The water content of the brain and the number of apoptotic cells were compared, Rusults The apoptosis of nervous cells was begun from 24 hour later and reached their peak level from 48 to 72 hours, To compare the NGF group with the injury group ,the declined of water content in brain were very obvious in 24, 48, 72 and 96 hours （P 〈0, 05） ; in while to compare the control group with injury group, the decline of water content were not obvious （ P 〉 0, 05 ） , Conclusions NGF has the obviously therapeutic effects on neuron apoptosis and brain edema after diffuse brain injury.
出处
《临床神经外科杂志》
CAS
2005年第4期151-152,共2页
Journal of Clinical Neurosurgery
关键词
脑损伤
凋亡
神经生长因子
brain injury
apoptosis
nerve growth factor
