摘要
目的研究阿魏酸钠(sodium ferulate,SF)对Aβ25-35引起的大鼠学习记忆障碍和海马CA1区锥体神经元损伤的保护作用。方法大鼠灌胃给予SF(50,100,250mg/kg)三周,脑室内注射凝聚态Aβ25-35(5μl,2mmol/L),2d后,进行Morris水迷宫定位航行实验,连续训练五天,第六天开始进行空间探索实验。行为学实验结束后,取脑组织,制成石蜡切片,进行尼氏(Nissl)染色和胶质纤维酸蛋白(glial fibrillary acidic protein,GFAP)免疫组织化学染色,研究海马CA1区锥体神经元形态学改变和星形胶质细胞的活化浸润情况。结果脑室内注射Aβ25-35可引起大鼠学习和记忆的障碍,表现为大鼠逃避潜伏期较对照组明显延长,大鼠在平台所在象限游泳距离百分比较对照组降低,这些行为学改变伴随着海马CA1区星形胶质细胞的浸润和锥体神经元的损伤。SF(50,100,250mg/kg)治疗组能剂量依赖的减轻Aβ25-35所致的学习记忆损伤,SF也能明显减轻海马CA1区锥体神经元的损伤和星形胶质细胞的浸润。结论阿魏酸钠能对抗Aβ25-35引起的海马锥体神经元的损伤和星形胶质细胞的活化浸润,从而改善大鼠的学习记忆能力。
Objective To study the protective effects of sodium ferulate on Aβ23-35 - induced cognitive deficits and damages of hippocampal CA1 pyramidal neurons in rats. Methods Rats were given SF （50, 100, and 250mg/kg） with ig method for 3 weeks, then, rats were injected intracerebroventricularly with Aβ23-35. Two days later, Morris water maze was used to measure the spatial learning and memory ability of rats. After behavior test, Nissl staining and immunohistochemical technique （for GFAP） were employed to determine the morphology of pyramidal neurons and astrocyte infiltration in hippocampal CA1. Results Intracerebroventricular injection of Aβ23-35 in rats resulted in spatial learning and memory impairments which shown by longer escape latency and decreased the percentage of swimming distant in the target quadrant. These behavioral dysfunction were accompanied by astrocyte activation and infiltration, the loss of pyramidal neurons in hippocampal CA1 .Oral administration of SF （50, 100, and 250mg/kg） markedly alleviated the memory impairment and the damage of hippocampal CA1 pyramidal neurons. Conclusions SF can improve the learning and memory ability of rats impaired by Aβ23-35 through suppressions of inflammatory response and protection of hippocampal CA1 pyramidal neurons.
出处
《锦州医学院学报》
2005年第5期1-4,共4页
Journal of Jinzhou Medical College
基金
辽宁省自然科学基金课题No.20042171
