摘要
目的观察非促分裂人酸性成纤维细胞生长因子(nmhaFGF)对大鼠视网膜缺血再灌注损伤后视网膜超氧化物歧化酶(SOD)、丙二醛(MDA)、一氧化氮(NO)的影响。方法40只SD大鼠随机分为对照组10只,实验组(nmhaFGF组)30只。实验组(大鼠左眼)又分为nmhaFGF低剂量组(1.25μg组)、nmhaFGF中剂量组(2.5μg组)、nmhaFGF高剂量组(5μg组)各10只,右眼为模型组(给予等量生理盐水)。采用升高眼内压的方法建立大鼠视网膜缺血再灌注模型。再灌注24h后分光光度法测定视网膜MDA、SOD含量,GRIESS反应测定NO含量。结果与模型组相比,nmhaFGF中剂量组、nmhaFGF高剂量组MDA含量显著降低(P<0.01),SOD、NO含量显著升高(P<0.05);nmhaFGF低剂量组和模型组之间SOD、MDA、NO含量没有显著差异(P>0.05)。结论nmhaFGF具有抗视网膜缺血再灌注氧自由基的作用;视网膜缺血再灌注损伤时,nmhaFGF可以增加视网膜内NO的含量,并呈现一定的量效关系。
Objective： To investigate the effect of non-mitogenetic human acidic fibroblast growth factor （nmhaFGF） injected into the vitreous cavity on superoxide dismutase （SOD）, malondialdehyde （MDA）. nitrogen monoxide （NO） after retinal ischemia-reperfusion injury. Methods： The rats were divided into control group, expermetal group. Experimental group were divided into nmhaFGF low dose group, nmhaFGF middle dose group and nmhaFGF high dose group randomly. After reperfusion 24h,the level of MDA and SOD were measured by spectrophotometer, the level of NO was measured by Griess method. Result.. Compared with the model, the content of MDA of high dose group and middle dose group was significant lower than the model group （P〈0.01）, and the contest of SOD and NO was significant higher than the model group （P〈0.01, 〈0.05）, but no such difference between low dose group and model group （P〈0.05）. Conclusion： Injection of nmhaFGF into the vitreous cavity has the antioxidation ability on experimental retinal ischemia-reperfusion injury, nmhaFGF increase the content of NO and it is in a dosage-dependent manner.
出处
《山东医药》
CAS
北大核心
2005年第34期15-16,共2页
Shandong Medical Journal
基金
国家863计划(2002AA2Z3318)
