摘要
目的:观察高纯度银杏黄酮对D-半乳糖所致衰老小鼠学习记忆功能的影响并探讨其作用机制,并与脑蛋白水解物的效果相比较。方法:实验于2004-11/2005-01在河北省职工医学院实验中心动物实验室进行,取ICR小鼠120只随机分为5组,每组24只:①银杏黄酮5,10mL/kg组:腹腔注射D-半乳糖400mg/kg,然后腹腔注射银杏黄酮5,10mL/kg。②脑蛋白水解物组:腹腔注射D-半乳糖400mg/kg和脑蛋白水解物注射液5mg/kg(0.4mL)。③模型组:腹腔注射D-半乳糖400mg/kg和生理盐水0.4mL。④正常对照组:腹腔注射生理盐水0.4mL。以上用药均共1次/d,连续8周。8周后跳台实验观察和测定各组小鼠学习记忆能力,然后颈椎脱臼处死小鼠,测定脑组织中乙酰胆碱酯酶、单胺氧化酶和超氧化物歧化酶活力及丙二醛含量。结果:经补充后120只进入结果分析。①跳台实验结果:银杏黄酮5,10mL/kg组和脑蛋白水解物组小鼠学习记忆训练时的错误次数均少于模型组(P<0.05),潜伏期长于模型组(P<0.05),但3组间比较无差异。②脑组织中乙酰胆碱酯酶活力:银杏黄酮5,10mL/kg组和脑蛋白水解物组低于模型组[(14.00±0.53),(11.00±2.90),(18.84±0.49),(24.50±1.96)nkat/g,P<0.05],但银杏黄酮5,10mL/kg组间比较无差异。③单胺氧化酶活力:银杏黄酮5,10mL/kg组和脑蛋白水解物组低于模型组[(14.49±2.50),(18.43±3.98),(22.32±4.86),(26.85±5.46)nkat/g,P<0.05],但3组间比较无差异。④超氧化物歧化酶、丙二醛水平:银杏黄酮5,10mL/kg组和脑蛋白水解物组高于模型组(P<0.05),但3组间比较无差异。结论:高纯度银杏黄酮治疗可明显改善D-半乳糖所致衰老小鼠学习记忆能力,其机制可能与增强脑组织乙酰胆碱及单胺类递质活性、清除自由基、抗脂质过氧化等作用有关。其作用与脑蛋白水解物相似,但不同剂量间无差异(5,10mL/kg)。
AIM： To observe the effects of high-purity ginkgo total terpenlactones on learning and memory function of senescent mice induced by D-galactose and explore its mechanism, and compare with the effects of brain protein hydrolysate. METHODS： The experiment was conducted at the animal laboratory of experimental center of Hebei Medical College for Staff and Workers from November 2004 to January 2005. 120 1CR mice were selected and assigned randomly into 5 groups with 24 mice in each group： ①5,10 mL/kg ginkgo total terpenlactones group： The mice were treated with 400 mg/kg D-galactose through intraperitoneal injection, and then injected with 5,10 mL/kg ginkgo total terpenlactones. ②Brain protein hydrolysate group： The mice were treated with 400 mg/kg D-galactose and 5 mg/kg （0.4 mL） brain protein hydrolysate through intraperitoneal injection. ③Model group： The mice were given 400 mg/kg D-galactose and 0.4 mL saline through intraperitoneal injection. ④Normal control group： The mice were given 0.4 mL saline through intraperitoneal injection. Above-mentioned medication was all once a day for 8 weeks. After 8 weeks, abilities of learning and memory of mice were observe and detected with jumping stand trial, and then the mice were killed with luxation on cervical vertebra. Activities of acetylcholinesterase, monoamine oxidase （MAO） and superoxide dismutase （SOD） and content of malondialdehyde （MDA） in tissues of brain were tested. RESULTS： After compensation, 120 mice were involved in the result analysis. ①Result of jumping stand trial： Mistake time when did learning and memory training in the 5,10 mL/kg ginkgo total terpenlactones group and brain protein hydrolysate group was less than that in the model group （P 〈 0.05）, and latency was longer than that in the model group （P 〈 0.05）, but there was no difference among the 3 groups. ②Activity of acetylcholinesterase in tissues of brain： It was lower in the 5,10 mL/kg ginkgo total terpenlactones group and brain protein hydrolysate group than that in the model group [（14.00±0.53）, （11.00±2.90）, （18.84±0.49）, （24.50±1.96）nkat/g,P 〈 0.05], but there was no difference in the 5, 10 mL/kg ginkgo total terpenlactones group. ③Activity of MAO： It was lower in the 5,10 mL/kg ginkgo total terpenlactones group and brain protein hydrolysate group than that in the model group [（14.49±2.50）, （18.43±3.98）, （22.32±4.86）, （26.85±5.46）nkat/g,P 〈 0.05], but there was no difference among the 3 groups. ④Levels of SOD and MDA： It was higher in the 5,10 mL/kg ginkgo total terpenlactones group and brain protein hydrolysate group than that in the model group （P 〈 0.05）, but there was no difference among the 3 groups. CONCLUSION： High-purity ginkgo total terpenlactones can improve significantly the abilities of learning and memory of senescent mice due to Dgalactose. Its mechanism can be related to the increase of effects of acetylcholine, activity of monoamine transmitter, clearance of free radical and anti-lipid peroxidation, etc. in tissues of brain. Its effect is similar to brain protein hydrolysate, but there is no difference in different dosage （5, 10 mL/kg）.
出处
《中国临床康复》
CSCD
北大核心
2005年第44期97-99,共3页
Chinese Journal of Clinical Rehabilitation
