摘要
目的:观察注射维生素C和芦丁后,急性疲劳性游泳运动小鼠机体各组织自由基代谢的变化情况。方法:实验于2004-09/12在泰山医学院基础医学研究所完成,选择雄性小鼠32只,随机分为4组,分别为生理盐水组、芦丁组、维生素C组和维生素C+芦丁组,每组8只。生理盐水组每天注射等量生理盐水,芦丁组注射芦丁5mg/d,维生素C组注射维生素C8mg/d,维生素C+芦丁组每天注射芦丁5mg和维生素C8mg,持续15d。各组小鼠进行池中游泳至力竭,力竭标准:动物不能在水中保持上浮,开始下沉,取出后尾部受刺激逃避呈踉跄步态,身体不能保持平衡,但呼吸心跳存在。麻醉后断头处死,测定小鼠脑、心、肝、肾和骨骼肌中丙二醛含量和超氧化物歧化酶活性分别采用硫代巴比妥酸比色法和氮蓝四唑光还原法。结果:所有小鼠全部进入结果分析,无脱失。①各组小鼠运动耐力比较:芦丁组、维生素C组及维生素C+芦丁组小鼠的耐力时间显著长于生理盐水组[(52.51±8.97,50.50±7.23和60.15±9.54,42.10±6.32)min(χ2=18.48～19.11,P<0.01)]。②各组小鼠各组织丙二醛/超氧化物歧化酶比值比较:芦丁组、维生素C组和维生素C+芦丁组小鼠心、肾、肝和肌组织中比值均显著低于生理盐水组(χ2=14.20～16.00,P<0.05),但维生素C+芦丁组的比值显著低于芦丁组和维生素C组(χ2=18.50～20.13,P<0.01)。结论:维生素C和芦丁均可降低丙二醛/超氧化物歧化酶比值,减少力竭运动后因脂质过氧化而产生的自由基,延长机体过度运动的耐力,但两者混合使用效果更好。
AIM： To observe the changes of metabolism of free radical of every constitution of acute tired swimming exercise mice after injecting with vitamin C and rutin. METHODS： The experiment was conducted at the institute of basic medicine of Taishan Medical College from September to December 2004. Thirty-two male mice were selected and randomly divided into four groups： saline group, rutin group, vitamin C group and vitamin C ＋ rutin group with 8 mice in each group. The mice in the saline group were treated with same volume saline every day. Those in the rutin group were given 5 mg rutin per day. Those in the vitamin C group were injected with 8 mg vitamin C per day. Those in the vitamin C ＋ rutin group were treated with 5 mg rutin and 8 mg vitamin C every day, lasting 15 days. The mice in every group swam in pool till exhausted. Standard of exhausting： The animals could not float in water, began to sink. After stimulating at the back tail, the animals escaped with staggering gait, and the bodies could not keep balance, but the breath and heartbeat existed. The mice were killed after anesthesia. Content of malondialdehyde （MDA） and activity of superoxide dismutase （SOD） in brain, heart, liver, kidney and skeletal muscle of mice were detected with thiobarbituric acid colorimetric method and nitroblue tetrazolium reduction, respectively. RESULTS： All the mice were involved in the result analysis, without drop-out, ①Comparison of exercise tolerance in mice of every group： Time of tolerance in mice of rutin group, vitamin C group and vitamin C ＋ rutin group was longer significantly than that in the saline group [（52.51±8.97, 50.50±7.23 and 60.15±9.54,42.10±6.32）min（χ^2=18,48-19.11 ,P 〈 0.01 ）]. ②Comparison of ratio of MDA/SOD of every constitution of mice in every group： The radios of heart, kidney, liver and muscle tissue in the rutin group, vitamin C group and vitamin C ＋ rutin group were all lower obviously than those in the saline group （χ^2=14,20-16.00,P 〈 0.05）. But it was lower markedly in the vitamin C ＋ rutin group than that in the rutin group and vitamin C group （χ^2=18.50-20.13,P 〈 0.01）. CONCLUSION： The vitamin C and rutin can decrease the ratio of MDA/ SOD, reduce level of free radical due to level of lipid peroxidation after exhaustive swimming and elongate tolerance of over-exercise, but the effect is better if use the mixture of the two.
出处
《中国临床康复》
CSCD
北大核心
2005年第44期132-134,共3页
Chinese Journal of Clinical Rehabilitation
