摘要
目的:比较肿瘤来源的热休克蛋白70(HSP 70)与白细胞介素2(IL-2)对荷瘤小鼠肿瘤切除术后的治疗作用。方法:液相色谱法纯化小鼠肿瘤细胞株中的HSP 70,用SDS PAGE及W estern b lot对纯化产物进行定性分析,毛细管电泳鉴定其纯度。动物实验观察HSP 70与IL-2对荷瘤小鼠肿瘤切除术后的治疗作用。结果:单纯手术能延长小鼠的生存期限,HSP 70 10μg组40%小鼠肿瘤最终全部消退,获长期生存(>90 d),平均生存(59.2±29.6)d,与对照组比较差异显著(P<0.05)。手术与HSP 70 10μg联合应用组80%肿瘤完全消退,平均生存(82.0±17.9)d,与对照组、单纯手术组、手术联合应用IL-2 10万u组比较差异显著(P<0.05,P<0.01,P<0.05)。结论:手术能切除局部肿物,但术后易复发、转移,影响长期生存率。HSP 70具有明显全身治疗作用,适当剂量的HSP 70能提高肿物切除后的荷瘤小鼠生存率,比术后应用IL-2治疗效果明显。
Objective： To compare the therapeutic efficacy of heat shock protein 70 （HSP 70） and interleukin-2 （IL-2） in tumor-bearing mice after tumor resection, and to provide evidence for combination therapy of surgery and HSP 70. Methods： HSP 70 was purified from the murine tumor cell line by using liquid chromatography and then identified by using SDS-PAGE and Western blot. The purity was analyzed by using capillary eleetrophoresis. The therapeutic efficacy of HSP 70 and surgery were observed in tumor-bearing mice. Result： Compared with the control group, the life span of tumor-bearing mice was longer in surgery alone group （P 〈 0.05）. In 10 lag HSP 70 group, 40% of tumors disappeared, and the mean life span was （59.2 ± 29.6） days. There was significant difference between the 10 lag HSP 70 group and the control group. In combination group of surgery and HSP 70, 80% of tumors disappeared, and the mean life span was （80.2 ± 17.9 ） days. Compared with the control group, surgery alone group, and combination group of surgery and IL-2, the life span was significantly longer in the combination group of surgery and HSP 70 （P 〈 0. 05）. Conclusion： HSP 70 has obvious systemic therapeutic effect. Combination therapy of surgery and appropriate dose of HSP 70 can increase the survival rate of tumor-bearing mice after tumor resection, and it is much better than the combination therapy of surgery and IL-2.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2005年第6期527-528,530,共3页
Journal of China Medical University
基金
辽宁省自然科学基金资助项目(2001102057)
