摘要
目的:研究红景天甙对人肝癌细胞系SMMC-7721的诱导分化作用。方法:通过不同剂量红景天甙处理体外培养的SMMC-7721细胞株,采用透射电镜观察细胞亚细胞结构、形态,放射免疫法、酚试剂法分别检测AFP与ALB分泌,重氮试剂法测定γ-GT活力,显微分光光度计定量测定细胞DNA。结果:红景天甙0.5 mg/ml1、.0 mg/ml1、.5 mg/ml均出现细胞增殖抑制,亚细胞结构趋于正常,AFP(alpha feto protein)分泌量下降(P<0.01,vs对照组),ALB分泌量增加(P<0.01,vs对照组),γ-GT(r-glulamgtranserpidage)活力下降(P<0.01,vs对照组),细胞DNA含量降低(P<0.01,vs对照组),并呈剂量效应关系。结论:红景天甙可影响SMMC-7721细胞结构、功能、代谢等指标,诱导其在体外向正常肝细胞分化。
Objective： To study the effect of salidroside on differentiation of SMMC - 7721 cell lines. Methods： By using the method of cell culture exposed to different doses of Salidroside,it was studied the effects of salidroside on SMMC - 7721 cell lines. The ultrastrueture of the cells was observed under transmission deetron microscope,the contents of AFP,ALB,γ - GT,DNA were detected by radioimmunity, phenol reagent, diazo reagent, microspectrophotometer, respectively. Results： The proliferation of treated cells was inhibited by salidroside, the ultrastrocture of the cells tended to normal, alpha feto protein（AFP） was lower, the activity of γ - glutamg - transperpidase （γ - GT） decreased gradually and the DNA content decreased, compared with those of the control cells Conclusion： Salidroside has effects on constitution, function and metabolism of SMMC- 7721 cell line and can induce the cell differentiation tending to normality.
出处
《西南国防医药》
CAS
2005年第6期613-615,共3页
Medical Journal of National Defending Forces in Southwest China
关键词
人肝癌细胞
诱导分化
红景天甙
human hepatoma call, differentiation, salidroside
