摘要
观音莲的顶芽或侧芽在MS+6-BA2.0 mg·L-1+TDZ0.03 mg·L-1培养基中,可诱导产生大量丛生泡叶状不定芽.以此作为增殖培养,每28 d增殖率达6.0以上.壮芽培养基宜用MS+6-BA0.3 mg·L-1+Y(多效唑)0.2 mg·L-1,周期30～35 d,由此获得的合乎生根标准的不定芽比率约80%左右.切取3 cm以上具完全展开叶的芽在MS+6-BA0.3 mg·L-1+Y(多效唑)0.2 mg·L-1培养基中,根的诱导率(生根率)达100%.
Alocasia amazonica top and lateral buds might be induced to produce large amount of fasclculate cystenchyma adventitious buds in the culture medium MS ＋ 6 - BA2.0 mg·L^- 1 ＋ TDZ0.03mg·L^- 1. The proliferation rate reached 6.0 and more every 28 days by taking it for the proliferation culture. The vigorous bud culture medium is suitable by MS ＋ 6-BA0.3 mg·L^- 1 ＋ Y0.2 mg· L^- 1, the cycle is 30～ 35 days long, the rate of adventitious bud obtained by the above way and suitable for the rooting standard was about 80 %. The root inducing rate was 100 % by cutting 3cm and longer of bud with wholly outspread leaf to put it into the culture medium MS＋ 6-BA0.3 mg·L^-1 ＋ Y0.2 mg·L^-1, the root inducing rate reached 100%.
出处
《福建林业科技》
北大核心
2005年第4期108-110,共3页
Journal of Fujian Forestry Science and Technology
关键词
组织培养
观音莲
tissue culture
Alocasia amazonica
