摘要
传统的蒽酮-硫酸分光光度法测定枸杞多糖质量分数时有偏差,为了准确地测定枸杞多糖的质量分数,对该法进行了全方位的系统研究。得到了最适宜的测定条件:吸收波长625．0nm,多糖溶液2mL,蒽酮-硫酸试剂4mL,水解显色10min．实验结果表明,改进后的蒽酮-硫酸法精密度高,平均回收率为98．93％,R=0．835％(n=6),能满足实验所需,是一种测定枸杞多糖质量分数的理想方法,亦可为其他类多糖质量分数的测定提供依据．
For the deviation occassionally occured in traditional anthrone-H2SO4 spectrophoto-metry method in determination of the polysaccharide content of Lycium barbarum, a proper measurement condition is proposed in order to improve the determination accuracy： 625.0 nm of absorption wavelength; 2.00 mL of polysaccharide solution; 4.00 mL of anthrone-H2SO4 reagent; 10 minutes of hydrolysis and chromogenic reaction time at room temperature. The test result shows that the determination accuracy is improved by the modified method, with average recovery rate of 98.93% （R=0.835%, n = 6）. It also can be referrences for other kind of polysaccharides mass fraction determination.
出处
《北京工业大学学报》
CAS
CSCD
北大核心
2005年第6期641-646,共6页
Journal of Beijing University of Technology
基金
北京市教育委员会基金资助项目(KP5800200352)
关键词
蒽酮-硫酸分光光度法
多糖质量分数的测定
枸杞多糖
anthrone-H2SO4 spectrophoto-metric
mass fraction determination of polysaccharide
Lycium barbarum polysaccharides
