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化学物质对硬壳蛤幼虫变态的诱导 被引量:9

Induction of metamorphosis of the hard clam Mercenaria mercenaria larvae by chemical cues
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摘要 用KCl、肾上腺素(EPI)、去甲肾上腺素(NE)、L-DOPA和GABA(γ-氨基丁酸)进行了不同浓度不同处理时间对硬壳蛤(Mercenaria mercenaria L.)幼虫变态诱导实验.结果表明,KCl、肾上腺素、去甲肾上腺素和L-DOPA对硬壳蛤幼虫的变态均有诱导作用,而GABA的诱导作用不显著.KCl的最佳诱导浓度随处理时间不同而有所不同.处理时间为1~24,48,72 h时KCl的最佳诱导浓度分别为33.56,20.13~26.85,13.42 mmol/L.肾上腺素和去甲肾上腺素的诱导作用与浓度和处理时间均有关.肾上腺素的最佳处理浓度为100 μmol/L,最佳处理时间均为8 h,此时幼虫变态率提高最大,为36.97个百分点.当去甲肾上腺素的诱导浓度为100 μmol/L,处理时间为8~16 h时,幼虫变态率提高也较大,均大于18个百分点,死亡率增加,但均低于30个百分点,当去甲肾上腺素诱导浓度为500 μmol/L时,虽然在8~16 h的处理时间范围内,幼虫变态提高率也较大,均大于18个百分点,但当处理时间超过8 h,在16~48 h范围内,幼虫死亡率提高明显增大,均大于50个百分点.L-DOPA的适宜诱导浓度为10~50 μmol/L,适宜处理时间为8~24 h,此时幼虫变态率均提高30个百分点以上,最高可提高79.43个百分点.GABA的诱导作用较弱,最佳诱导浓度随处理时间的不同而有所不同,处理时间为24 h和48 h时,最佳诱导浓度为0.1 μmol/L;处理时间为0.5~16 h时,最佳诱导浓度为100 μmol/L. The abilities of KCI, epinephrine (EPI), norepinephrine (NE), L-DOPA, and GABA (γ-aminobutyric acid) to induce metamorphosis of Mercenaria mercenaria L. larvae were studied. Results suggested that KCI, EPI, NE, L-DOPA were active inducers of metamorphosis, whilst GABA was less effective. Optimum concentration of KCI varied with different exposure time, and was 33.56 mmol/L when the exposure time was 1-24h, with metamorphosis rate increments all 〉24 percentage. The optimum concentrations of KCI were 20.13-26.85 mmol/L and 13.42 mmol/L when the exposure time lasted 48h and 72 h, respectively. The inductive effects of EPI and NE were dose- and time-dependent. The optimum concentration of EPI was 100 μmol/L at 8h exposure time, with the highest metamorphosis rate increment being 36.97 percentage. The metamorphosis rate increment was〉18 percentage when the larvae were exposed to 100 μtmol/L NE for 8-16h, with the mortality rate increment all〈30 percentage. Exposure to 500μmol/L NE for 8-16 h was also sufficient for the larvae to increase metamorphosis rate〉18 percentage, but prolonged exposure time (16-48 h) caused toxic effect, with the mortality rate increasing significantly, all〉50 percentage. The better inductive effects of L-DOPA were archived when the larvae were exposed in 10μmol/L and 50/,mol/L for 8-24 h, with the metamorphosis rate increment〉30 percentage.
出处 《海洋科学》 CAS CSCD 北大核心 2005年第12期59-67,共9页 Marine Sciences
基金 国家"863"项目(2004AA603810) 国家自然科学基金项目(30200214) 国家级星火计划项目(2004EA741014) 山东省科技兴海项目"硬壳蛤工厂化育苗与养殖产业化开发" 青岛市重大科技项目"硬壳蛤工厂化人工育苗与示范"
关键词 化学物质 硬壳蛤(Mercenaria MERCENARIA L) 变态 诱导 chemical cues Mercenaria mercenaria metamorphosis induction
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