摘要
评价南方膳食结构中铁的吸收率,为我国不同人群、不同膳食类型的铁吸收率提供参考资料。采用^(57)Fe-EDTA或^(57)FeSO4两种铁的稳定性同位素示踪方法,以稀土元素Dy作为排泄物的回收标记物,于现场进行人体代谢试验,受试者为健康成年男子和孕龄妇女各10名,分6次口服3.0mg的^(57)Fe稳定性同位素示踪剂及2.0mg的Dy,粪便监测法收集同位素排出粪样;用电感耦合等离子体质谱仪测定粪样^(57)Fe/^(56)Fe的比值,用原子吸收光谱法测定粪样总Fe,从而计算该人群膳食铁的吸收率。在南方膳食结构中,口服^(57)Fe-EDTA示踪剂,成年男子膳食铁的吸收率为7.34%,孕龄妇女的吸收率为6.92%;口服^(57)Fe-SO4示踪剂,成年男子膳食铁的吸收率为6.01%,孕龄妇女的吸收率为5.40%。本研究结果表明口服^(57)Fe-EDTA评价膳食铁吸收率虽有高于用^(57)FeSO4评价结果的趋势,但无显著性统计分析差异。中国南方城市膳食中铁的吸收率与2000年中国营养学会对我国居民膳食铁吸收率的估计水平大致相当。
The iron （Fe） absorption in the dietary pattern of the cities of South China was evaluated by enriched stable isotope ^57 iron tracer method with ^57Fe-EDTA and ^57FeSO4 as tracers and the rare earth element dysprosium （Dy） as the marker indicating the tracer recovery. Ten healthy men and 10 healthy women were recruited as subjects in-situ metabolic trial taking 3.0mg Fe tracer and 2.0mg Dy in six meals of the continuous 2days. Fecal samples were collected by fecal monitoring; The isotopic ratio of ^57Fe/^56Fe was measured by inductively coupled plasma mass spectrometry （ICP-MS） and the total Fe was detected by atomic absorption spectrometry （AAS）. In the dietary pattern of South China, taking ^57 Fe-EDTA as tracer, the iron absorption for the men and women were 7. 34 % and 6.92% ,respectively,while for 57FeSO,, 6.01% and 5.40% were obtained. The iron absorption from ^57 Fe-EDTA was likely higher than that from ^57 FeSO,, but having no statistic difference, and comparable with level evaluated by the nutrition societv of China in 2000.
出处
《光谱实验室》
CAS
CSCD
2005年第6期1132-1137,共6页
Chinese Journal of Spectroscopy Laboratory
基金
深圳市科技计划项目(200304162)
