摘要
中药材对后天环境依赖性很强因而质量不稳定,指纹图谱是中药质量控制的重要手段,本文提出用高速逆流色谱 (HSCCC)法研究中药指纹图谱。用高速逆流色谱仪分离纯化3个批次的丹参脂溶性成分,选用正己烷-乙醇-水(10:5．5: 4．5)体系,进行分步洗脱,固定相保留率达到78．8％,保证了系统的分辨率。3个丹参样品分剐分离得到12个洗脱组分,经检测3份洗脱图谱中对应洗脱峰为同一组分,洗脱峰保留时间的相时标准偏差RSD均<3％;不同批次样品中同一组分的相对含量有较大差异,说明HSCCC法可以体现中药化学成分浓度分布的状况。利用液-质联用仪将洗脱组分与3种主要活性成分的标准对照品进行比对,标定出指纹图谱中的峰7,8和II分别为隐丹参酮(Mw296．33)、丹参酮Ⅰ(Mw276．27)和丹参酮ⅡA(Mw294．19)。研究表明HSCCC法作为指纹图谱研究方法具有可行性。
Active constituents of the traditional Chinese medicine（TCM）are influenced by soils,climates,and growth stages. Therefore,fingerprint is important in quality control of TCM. Our studies showed that HSCCC could be applied in the development of fingerprint of TCM. Twelve Components were separated from extracts of 3 crude samples of Salvia mihiorrhiza Bunge,which were collected from different growth locations. HSCCC was performed with n-hexane-ethanol-water（10 ： 5.5 ： 4.5,v/v）in gradient mode. Retention of the stationary phase was 78.8% ,which assured the resolution of separation. The three samples were found to contain 12 fractions on HSCCC,with similar retention time. However contents of the corresponding fractions in each sample were quite different,which showed that HSCCC could express the concentration distribution of the constitutents. Major active constituents were identified in the fingerprint of S. mihiorrhiza Bunge by liquid chromatographymass spectrometer. It was concluded that fraction number 7,8 and 11 were respectively crypototanshinone （Mw 296）, tanshinone[ （Mw 276）and tanshinoneⅡ A（Mw 29,1 ）. The study demonstrated that HSCCC was a feasible method for fingerprint of TCM.
出处
《中国药品标准》
CAS
2005年第6期7-12,共6页
Drug Standards of China
关键词
中药指纹图谱
高速逆流色谱法
丹参
活性成分标定
fingerprint of traditional Chinese medicine,High-speed counter-current chromatography（HSCCC）,Salvia miltiorrhiza Bunge ,identification of active constituent
