摘要
优化了中国樱桃品种“对樱”不定根离体再生体系,用农杆菌介导法,将抗菌肽B基因导入“对樱”,通过抗性筛选获得了44株抗性转化植株。PCR检测有9株为阳性,初步说明抗菌肽基因已整合到“对樱”基因组中。
The system of regeneration in vitro from adventitious roots in Chinese cherry （Prunus pseudocerasus Lindl. cv. Duiying） was optimized. The antibacterial peptide B gene was transformed to cherry with Agrobacterium tumefaciens-mediated transformation system. The 44 plants regenerated which had resistance to Gent were deteced by PCR, and 9 plants were positive. It indicated into the genome of cherry. that the antibacterial peptide gene had been integrated
出处
《植物生理学通讯》
CSCD
北大核心
2005年第6期770-772,共3页
Plant Physiology Communications
基金
北京市自然科学基金重点项目(6041002)
关键词
中国樱桃
抗菌肽
转基因
Chinese cherry （Prunus pseudocerasus Lindl. cv. Duiying）
antibacterial peptide
genetic transformation
