摘要
以壳聚糖分别与硫酸软骨素、羧甲基壳聚糖、透明质酸按一定比例制备出共混膜,研究了膜片的透光性、吸水率、渗透性、力学性质、表面结构、红外图谱等物理性质。以共混膜为载体培养兔角膜内皮细胞,从而筛选出了透明度高,亲水性好,机械性能优异,最适合内皮细胞贴附生长的壳聚糖-硫酸软骨素共混膜片,并对其生物降解性、生物相容性等性质进行了研究。为了提高壳聚糖-硫酸软骨素共混膜片的通透性,在膜片制备过程中加入不同剂量的醋酸盐作为扩孔剂,结果表明当加入一定量的醋酸盐为扩孔剂时,膜片的通透性提高。
Three kinds of blend films were prepared by （1）chitosan and chondroitin sulfate, （2）carboxymethyl- chitosan and （3） hyalurnnic acid, respectively. Transparency, water absorption percentage, permeability, mechanical properties, SEM, FT-IR of the blend films were analyzed. Rabbit corneal endothelial cells were cultured on the blend films. Chitosan-chondroitin sulfate blend film was screened as the best scaffold for the attachment and growth of corneal endothelial cell. Biodegradability and biocompatibility of chitosan-chondroitin sulfate blend film were studied. In order to improve the permeability of chitosan-chondroitin sulfate blend film, different dosage of acetate was added during the preparation of film, the results indicated that the permeability was improved when certain dosage of acetate was added.
出处
《中国生物医学工程学报》
EI
CAS
CSCD
北大核心
2005年第6期755-762,共8页
Chinese Journal of Biomedical Engineering
基金
国家"十五"863资助课题(2003AA625050)
国家自然科学基金资助项目(30070220)
关键词
壳聚糖
共混膜
物理性质
生物相容性
角膜内皮细胞
chitosan
blend film
physical properties
biocompatibility
corneal endothelial cell
