摘要
目的:用胃肠癌冻融抗原冲击树突状细胞(DC)诱导荷瘤小鼠脾细胞,观察DC及细胞毒T细胞(CTL)的生物学功能及对胃肠癌靶细胞体外杀伤作用。方法:小鼠骨髓细胞经GM-CSF、IL-4诱导和胃肠癌细胞系KATO3、CT26细胞冻融抗原(Ag)的冲击,诱生致敏的DC与活化脾淋巴细胞共培养,获得的CTL对相应靶细胞及对照黑色素瘤细胞进行体外杀伤。结果:制备的DC与CTL具有良好的生物学活性,胃肠癌Ag冲击的DC诱导的CTL对相应靶细胞的杀伤率分别平均75.98%和86.75%,且效靶比显示量效关系;对无关靶细胞A375为10.57%和11.53%。结论:胃肠癌Ag冲击致敏的DC能诱导CTL产生特异性杀伤作用。
Objective： To observe the activity of dendritic cells （DC） against mouse gastric-intestinal cancer in vitro used DC pulsed with lysates of gastric-intestinal tumor cell （TAg） to induced murine spleen cells. Methods： DC, derived from murine bone marrow was induced by combination of GM-CSF, IL-4, and pulsed with TAg lysates of KATO3 and CT26 and then DCs were cocuhured with spleen cells for 3d. And then these cytotoxic T cells（CTL） were used to kill the KATO3, CT26 and A375 target cells and the killer activities were detected by flow cytometry（FCM） and MTT method. Results： The biological activities of DC and CTL were detected well by FCM, and the cytotoxic activities of CTL induced by DC pulsed with TAg of KATO3 and CT26 were 75.98% and 86.75% to A375 target cells they were 10.57% and 11.53%. Conclusion： The CTL induced by DC pulsed with relative TAg has a significant specific cytotoxicity role to gastric-intestinal cancer target cell line cells in vitro.
出处
《军医进修学院学报》
CAS
北大核心
2005年第6期468-470,共3页
Academic Journal of Pla Postgraduate Medical School
关键词
树突状细胞
细胞毒T细胞
胃肠肿瘤
冻融抗原
dendritic cell
cytotoxic T cell
gastric intestinal cancer
lysates antigen
