摘要
探讨HCV感染者外周血淋巴细胞亚群与其基因型、肝脏炎症以及与干扰素α(IFN-α)治疗的相关性。用流式细胞仪和限制性片段长度多态性(RFLP)对41例HCV感染者外周血淋巴细胞亚群百分比及HCV基因分型进行分析。未经治疗患者的激活T细胞(CD3+HLA-DR+)、激活CD4+T细胞(CD4+HLA-DR+、CD4+CD25+)及NK 细胞(CD3-CD16／CD56+)均低于正常(P<0．05);CD8+T(CD8+CD3+)细胞、激活(CD8+T细胞(CD8+HLA-DR+、 CD8+CD38+)及B细胞(CD19+)高于正常(P<0．05);使用IFN治疗后显著提高了患者外周血激活T细胞激活 CD4+T细胞及NK细胞百分比(P值<0．05);同时降低CD8+T细胞、激活CD8+T细胞及B细胞百分比(P<0．05)。此外,还发现在伴有肝脏炎症患者的CD8+细胞、激活CD8+T细胞要明显高于不伴有炎症的患者(P<0．05),而NK 细胞则要显著低于不伴有炎症者(P<0．05);不同HCV基因型感染者外周血淋巴细胞亚群百分比均无显著性差异 (P>0．05)。
To discuss the relation of HCV genotype, IFN- alpha treatment, liver IFN lamination and the lymphocyte subset of peripheral blood of HCV sufferer. We examined subsets of blood lymphocytes using flow cytometry, and identified the genotype of HCV by RFLP. After IFN- alpha therapy there are a significant increase in subset of CD3^＋ HLA - DR^＋ ,CD4^＋ HLA - DR^＋ , CD4^＋ CD25^＋ and NK cell（CD3 - CDI6/CD56^＋ ）（ P 〈 0.05） and significant decrease in subset of CD8^＋ CD3^＋ ,CD8^＋ HLA - DR^＋ ,CD8^＋ CD38^＋ and B cell （CD19^＋）（P〈 0.05）. The percentage of CD8^＋ CD3^＋ ,CD8^＋ HIA- DR^＋ and CD8^＋ CD38^＋ in case with hepatitis is higher than in case without hepatitis; but The percentage of NK cell（ CD3 - CD16/CD56^＋ ） in case with hepatitis is lower than in case without hepatitis. The genotype of HCV has nothing to do with the lymphocyte subset of peripheral blood of HCV sufferer.
出处
《临床肝胆病杂志》
CAS
2005年第6期347-349,共3页
Journal of Clinical Hepatology
