摘要
目的检测原发性胆汁性肝硬化(primarybiliarycirrhosis,PBC)患者外周血CD4+CD8+T、CD4+CD25+调节性T细胞亚群,探讨其与肝功能损伤、抗AMAM2抗体产生的关系。方法采用流式细胞术检测北京协和医院住院和门诊PBC患者(n=27)外周血CD4+CD8+T细胞、CD4+CD25+T细胞群比例,以26例其他肝脏疾病患者作为疾病对照组,30例健康体检者作为正常对照,观察调节性T细胞亚群与患者的肝功能指标及自身抗体如AMAM2、ANA的关系。结果PBC患者外周血CD4+CD25+T细胞比例与正常对照组比较结果差异无统计学意义(P>0.05),但显著高于其他肝脏疾病组(P<0.05)。CD4+CD8+T细胞群与正常人相比结果差异无统计学意义,其他肝病组明显高于正常对照组(P<0.05)。PBC患者外周血NK细胞比例显著低于正常对照组(P<0.05)。肝功严重损伤的PBC患者外周血CD4+CD25+T细胞比例明显高于肝功能轻度损伤者(P<0.05)。PBC患者外周血CD4+CD25+T细胞数量与肝功能损伤指标ALB呈负相关(r=-0.338,P<0.05),与TBIL、DBIL呈正相关(r分别为0.362,0.386,P<0.05)。CD4+CD25+/CD4+比例与GGT呈负相关(r=-0.335,P<0.05),与TBIL、DBIL呈正相关(r分别为0.333,0.339,P<0.05)。抗AMAM2抗体阳性患者外周血CD4+CD25+细胞比例明显高于AMAM2阴性患者(P<0.01)。未发现CD4+CD25+T、CD4+CD8+T细胞比例在ANA+和ANAPBC患者之间差异有统计学意义。结论PBC患者外周血CD4+CD25+T细胞群比例与肝功能损害和抗AMAM2抗体的产生密切相关,因此推测CD4+CD25+T细胞的变化可能是导致病情发展以及肝脏损伤的关键环节之一。
Objectives To investigate the ratios of peripheral blood CD4＊ CD8^＋ and CD4^＋ CD25^＋ regulative T cells, and explore the association with hepatic damnifieation and anti-AMA-M2 antibodies. Methods The percentage of CD4^＋ CD8^＋ T cells and CD4^＋ CD25 ^＋ T cells in peripheral blood from patients with primary biliary cirrhosis （ PBC ） （ n = 27 ）, 26 patients with other hepatic desease, 30 normal individuals were measured by floweytometry. Results Patients with PBC had statistically higher levels of CD4^＋ CD25^＋ T cells than the patients with other hepatic disease （ P 〈 0. 05 ）. No significant could be found between patients with PBC and normal controls. Patients with PBC had statistically lower levels of NK cells than the normal controls （ P 〈 0.05）. The percentage of peripheral blood CD4^＋ CD25^＋ T cells in patients with severe hepatic damnifieation was significantly higher compared to patients without severe hepatic damnification（ P 〈 0.05 ）, and related to hepatic damnification. Patients with positive anti-AMA-M2 antibody still had significantly higher levels of CD4^＋ CD25^＋ T cells than the patients with negative anti-AMA-M2 antibody （P 〈 0. 01 ）. No significant could be found between patients with positive ANA antibody and patients with negative ANA antibody. Conclusions The increase of peripheral blood CD4^＋ CD25 ^＋ T cells inpatients with primary biliary cirrhosis is related to hepatic damnification and also associate with AMA-M2 antibody. CD4^＋ CD25 ^＋ T cells may play a crucial role in the controlling of disease progressing and hepatic damnification.
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2005年第12期1243-1247,共5页
Chinese Journal of Laboratory Medicine
基金
国家自然科学基金资助项目(30471617)
国家863高技术计划资助项目(20024032)
