摘要
目的NOR1基因真核表达载体pcDNA3.1(+)的构建并了解其对肝癌细胞系HepG2生长的影响。方法将NOR1cDNA亚克隆至pcDNA3.1(+)真核表达载体上,并把重组质粒pcDNA3.1(+)/NOR1经脂质体转染至HepG2细胞中,运用MTT法、台盼蓝排斥等试验、流式细胞仪等分析NOR1基因对肝癌细胞HepG2生物学活性的影响。结果成功构建了NOR1基因真核表达体pcDNA3.1(+)/NOR1,经pcDNA3.1(+)/NOR1转染后的HepG2细胞生长速度明显抑制(P<0.05),且细胞从G0/G1期进入S期明显延缓。结论重组质粒pcDNA3.1(+)/NOR1能在HepG2细胞内表达,且能影响HepG2细胞的生长。
Objective To construct the eukaryotic expression vector of pcDNA3. 1 （ ＋ ）/NOR1 and analyze the effect of NOR1 on the liver cancer cells. Methods NOR1 cDNA was cloned into eukaryotic expression vector pcDNA3. ! （ ＋ ）, recombinant eukaryotic expressing plasmid pcDNA3. 1 （ ＋ ）/NOR1 was transiently introduced into human liver cancer cell line HepG2 mediated by cation iron lipofectamin. The biology effect of NOR1 on the liver cancer cells was analyzed through the MTT test, trypan blue exclusion assay and flowcytometric analysis. Results The eukaryotic expression vector of pcDNA3. 1 （ ＋ ）/NOR1 was successfully constructed. The liver cancer cell growth rate was obviously slow after it was transfected by recombinant plasmid pcDNA3.1 （＋）/NOR1 and the cell cycle from G0/G1 to S distinctly prolong. Conclusions Recombinant plasmid pcDNA3. 1 （ ＋ ）/NOR1 can express in HepG2 cells and affect the growth of HepG2 cells.
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2005年第12期1267-1269,共3页
Chinese Journal of Laboratory Medicine
基金
国家自然科学基金资助项目(30300383)
