摘要
目的探讨脂多糖(LPS)对THP-1细胞CD14诱导表达的影响.方法应用不同质量浓度(0.1～50 mg/L)的LPS刺激培养48 h的THP-1细胞,并测定其CD14mRNA、肿瘤坏死因子α(TNFα)和白细胞介素6(IL-6)表达的变化.结果正常培养条件下,THP-1细胞CD14表达量极低,而LPS对单核巨噬细胞THP-1具有显著的诱导激活作用,在较低质量浓度剂量刺激下即可被活化,表现为CD14mRNA表达量迅速增加,同时,LPS可诱导THP-1细胞产生TNF-α和IL-6,并在一定范围内呈剂量依赖关系.结论 LPS可导致THP-1细胞活化,显著上调CD14mRNA的表达,并诱导产生炎症介质TNF-α和IL-6.
Objective To investigate the effects of LPS on expression of mCD14 on the THP-1 cell line. Methods Under vitro cultured conditions human THP-1 cell line was stimulated respectively by 0（as control）, 0.1-50 mg/L LPS for 24 h, the cell apoptosis rate was determined with flow cytometry. The contents of TNFa and IL-6 in the supematant were measured by ELISA. The expression levels of mCD14 on the cell membrane were measured in the levels of relative density of PCR products. Results After activation of LPS, the cell apoptosis rate was promoted and levels of TNF-α and IL-6 in culture supernatant were rernarkablely increased. The mCD14 expression on the cell membrane was significantly up-regulated. Conclusion LPS can lead to activition of THP-1 and significantly up-regulate the expression of CD14mRNA. It reveals that LPS may be an important inflammatory factor on THP-1 cell reactions.
出处
《首都医科大学学报》
CAS
2005年第6期711-713,共3页
Journal of Capital Medical University
基金
北京市科技新星计划项目(H013610290113)
北京市委组织部优秀人才培养专项经费资助项目
