摘要
本文以PK-15细胞为模型,研究了亚硒酸钠、硒蛋氨酸和海藻硒多糖等三种硒化合物对猪细小病毒体外复制 的抑制作用,以及还原型谷胱甘肽、D-甘露醇等氧自由基清除剂对不同来源硒的抑制病毒作用的影响。结果表明: 三种硒化合物对猪细小病毒在PK-15中的复制呈现不同程度的抑制作用,在相同浓度时其强度依次为硒蛋氨酸、 亚硒酸钠、海藻硒多糖,随着浓度的增加,其抑制作用逐渐增强,呈剂量依赖性关系。还原型谷胱甘肽和甘露醇均 有增强硒的抑制病毒复制作用,两者同时添加时,协同增强硒的抑制病毒复制作用。
Inhibitory effects of sodium selenite, DL-selenomethionine and Kappa-selenocarrageenan on Porcine parvovirus （PPV） infection of the PK-15 cells were studied. The influences of reduced glutathione （GSH） and D-mannitol on antiviral effects of selenium were also carried out in PK-15 cells. The results showed that all of the three selenium sources have an inhibitory effect on the PPV replication in vitro. DL-selenomethionine had the highest inhibitory effect while Kappa-selenoearrageenan had the lowest, and the inhibition was concentration-dependent. The GSH and D-mannitol have the ability to increase the antiviral effects of selenium. The antiviral effect was enhanced with the addition of both GSH and D-mannitol.
出处
《中国病毒学》
CSCD
2005年第6期613-617,共5页
Virologica Sinica
基金
国家自然科学基金资助项目(30371060)河南省重大科技攻关项目(0223013800)
关键词
猪细小病毒
亚硒酸钠
硒蛋氨酸
海藻硒多糖
还原型谷胱甘肽
甘露醇
Porcine parvovirus
Sodium selenite
Kappa-selenocarrageenan
DL-Selenomet hionine
Glutathione reduced
D-Mannitol
