摘要
研究了分属3个市场型的10个中国花生栽培种的序列相关扩增多态性(SRAP)。结果表明, SARP技术可以揭示花生栽培种的遗传差异。在所试验的74对SRAP引物中,2对只获得了单态性条 带,其余72对总共产生了1,812条带,其中1035条(57．12％)为多态性带,每对引物组合平均获得25．16 条带、14．38条多态性带。在这73对引物组合中,总带数和多态性条带的数目分别为7-63和2-44 条。10个花生栽培种的简单匹配相似系数为0．6967-0．9171不等,根据SRAP指纹图谱进行聚类分析 可将这10个品种分为5类。用64对引物筛选作图亲本24-3与B4,获得329条多态性条带。此项研究 为花生品种鉴定和遗传研究提供了新的DNA分子标记技术手段。
SRAP assay was conducted using 10 peanut cuhivars from China of 3 market types and 51 SRAP primer combinations to evaluate the utility of SARP in peanut. Among the 74 primer pairs tested, 2 produced mono-morphie bands only, and seventy-two totally produced 1,812 bands, among which 1035 （57.12%） were polymorphie, with an average of 14.38polymorphic bands and 25.16 bands per primer combination. The total number of bands and polymorphie bands in the 74 primer combinations ranged from 7-63 and 2-32 respectively. The simple matching coefficient of＇the peanut varieties ranged from 0.6967 to 0.9171. The varieties may be grouped into 5 categories based on cluster analysis of the SRAP tqngerprinting data. In a separate study on SARP using 64 primer pairs, 329 polymorphie bands were detected in 24-3 and B4, the parental genotypes for mapping. This study provided an additional marker system for peanut variety identification and genetic research.
出处
《中国农学通报》
CSCD
2005年第12期35-39,共5页
Chinese Agricultural Science Bulletin
基金
国家973前期专项"缺项目名称-优质花生分子标记的基础研究"(2002ccc03200)国家863计划项目"优质高产专用花生新品种培育技术研究与利用"(2002AA207010)"优质
高产
多抗
专用花生和芝麻新品种培育"(2004AA241150)国家自然科学基金项目"花生SSR
AFLP标记的开发及分子连锁框架图构建"(30300224)青岛市科技计划项目"优质花生分子标记的研究"(02-1-kj-yj-48)
