摘要
目的 应用光子计数的方法在CuSO4-Phen-VC-H2O2-DNA反应体系中比较反式和顺式白藜芦醇淬灭羟自由基的能力。 方法 用RP-HPLC从虎杖药材中收集白藜芦醇的反式异构体。在254 nm紫外光下诱导18 min,使所得反式异构体组分发生 异构化,生成顺式异构体。在相同色谱条件下,收集顺式异构体。CuSO4-Phen-VC-H2O2-DNA反应体系可产生羟自由基并释放 光子。应用反式和顺式两种异构体组分在该体系中淬灭羟自由基。结果 反式和顺式两种异构体组分在该体系中淬灭羟自 由基的最小用量分别为1和10mg·L-1。超过该值,两个组分均能明显降低反应体系的最大光子计数值,并延迟最大光子计数 值出现的时间。结论 反式异构体淬灭羟自由基的能力比顺式异构体强。
OBJECTIVE To develop a HPLC ashy for determing the E-isomer fraction of resveratrol collected from a traditional Chinese herb, Polygorum cuspidatum Sieb, et Zucc. in this study.METHODS Collected E-isomer fraction was exposed to UV light （254 nm） for 18 rain to induce the formation of Z-isomer. Z-isomer fraction was consequently purified by RP-HPLC under the same conditions as E-isomer purification. Hydroxyl radical （-OH） was generated from CuSO4-Phen-VC-H2O2-DNA reaction and was extinguished by both of fractions. The number of photon generated from the reaction was counted by an ultra weak luminescence analyser （ photon counter）. RESULTS one mg·L^-1 E- and 10mg·L^-1 Z-resveratrol, respectively, were the limits to quench hydroxyl radical. Over those amounts,both of fractions were able to reduce the maximum photon number significantly and the maximum photon number appeared later. CONCLUSION This observation also suggests that E-isomer has a stronger ability to quench radical than Z-isomer did.
出处
《中国药学杂志》
EI
CAS
CSCD
北大核心
2005年第24期1892-1894,共3页
Chinese Pharmaceutical Journal
关键词
白藜芦醇
自由基
resveratrol
free radical
