期刊文献+

水蛭素滴眼液的研究 被引量:4

Studies on ophthalmic preparation of Hirudin
下载PDF
导出
摘要 目的:制备水蛭素滴眼液,考察其稳定性及其对白内障防治作用。方法:以溶红细胞试验筛选促吸收剂,用正交试验(L9(33))确定水蛭素滴眼液的处方组成。选用硒性白内障和半乳糖性白内障大鼠动物模型考察水蛭素滴眼液对白内障的防治作用。结果:在pH7.4硼酸缓冲液中,以0.5%EDTA和0.5%壳聚糖作为该滴眼液的促吸收剂。该制剂在4℃冰箱和室温放置12个月,其抗凝活力,外观和pH值均无改变。本制剂对眼无刺激性,减轻晶状体浑浊程度,降低MDA含量,增大SOD活力,增加γ蛋白含量。结论:本制剂性质稳定,对硒性及半乳糖性大鼠白内障有显著的防治作用。 Objective:To investigate the preparation, stability and effect of Hirudin eyedrops for the treatment of cataract in rats. Methods:The absorption promoters were screened using globulolytic test; the formulation was optimized by orthogonal design L9 (3^3);the rat cataract modules induced by selenium and galactose were used as tools to evaluate the efficacy of cataract treatment. Results: In the boric acid buffer solution (pH 7.4), 0.5 % EDTA and 0.5 % chitosan were considered to be the optimal absorption promoters The formulation was stable at 4℃ and ambient temperature for 12 months, i.e., no changes of antithrombin actlvitv, appearance and pH value. The ophthalmic fornullatinn showed no irritation to rat' s eyes. The Hirndin rats experienced regression of the lens opacities, decrease of MDA concentrations and increase of SOD activity and γ protein levels. Conclusion: The stable ophthalmic preparation of Hirudin could manage the cataract induced by selenium and galactose in rats .
出处 《中国新药杂志》 CAS CSCD 北大核心 2005年第11期1308-1311,共4页 Chinese Journal of New Drugs
基金 教育部博士学科点专项基金(20040316005)
关键词 水蛭素 滴眼液 晶状体 稳定性 白内障 hirudin eye drop len stability cataract
  • 相关文献

参考文献7

二级参考文献10

共引文献28

同被引文献108

引证文献4

二级引证文献22

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部