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高效液相色谱法测定食品中齐墩果酸的含量 被引量:2

Determination of Content of Oleanolic Acid in Mung Bean, Red Bean, Lotus Seed and Jujube Respectively by HPLC
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摘要 采用高效液相色谱法对绿豆、赤豆、莲子、枣中的齐墩果酸进行分离和分析,所用色谱柱为NucleodurC18柱(250mm×4.6mmi.d.,5μm);流动相:80%乙醇;在选定色谱条件下其齐墩果酸的线性范围良好,样品加样回受率在96.7%~101.5%,相对标准偏差在0.64%~2.89%。 Oleanolic acid was important bioactive components in mung bean, red bean, lotus seed and jujube. The contents of these compounds in food were determined by reversed-phase high performance liquid chromatography. Nucleodur C18 column (250mm × 4.6mmi.d., 5μm)was used with ethanol-water (80:20) as the mobile phase. The good linear relationships were obtained under the optimum conditions for each component. The recoveries of the standards added were 96.7%-101.5%, while the relatively standard deviations were within the range of 0.64%-2.89%.
作者 吴波 张寒俊
出处 《食品科学》 EI CAS CSCD 北大核心 2005年第12期176-178,共3页 Food Science
关键词 高效液相色谱 齐墩果酸 食品 绿豆 赤豆 莲子 high performance liquid chromatography oleanolic acid food mung bean red bean lotus seed jujube
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