摘要
L苯丙氨酸因其在工业、医药上的重要作用而日益受到人们的重视.苯丙氨酸解氨酶(PAL)生物转化肉桂酸生产L苯丙氨酸成了该领域研究与开发的热点.本文利用简并寡核苷酸PCR法结合cDNA末端快速扩增PCR法,从粘红酵母中克隆了苯丙氨酸解氨酶cDNA核心序列,为克隆其cDNA全长序列及体外表达其活性蛋白奠定了基础.
L-phenylalanine is now an important amino acid for food and medicine industries. The biotransformation of transcinnamic acid to L-phenylalanine by using phenylalanine ammonia-lyase （PAL） has, therefore, become very important in the research and development of this field. In this paper the gene encoding PAL was cloned by degenerating oligo primed polymerase chain reaction （PCR） and random amplification of cDNA end （RACE） PCR procedure, and a core PAL cDNA sequence cloned was analyzed from Rhodotorula glutinis CIBAS A 1401. Fig 5, Ref 22
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2005年第6期694-698,共5页
Chinese Journal of Applied and Environmental Biology
基金
浙江亚美生化股份有限公司资助
江苏常茂生物化学工程股份有限公司资助~~
关键词
粘红酵母
苯丙氨酸解氨酶
基因克隆
CDNA
Rhodotorula glutinis CIBAS A 1401
phenylalanine ammonia lyase
gene cloning
cDNA
