摘要
目的观察25％氯硝柳胺悬浮剂对日本血吸虫尾蚴的杀灭效果,确定杀蚴有效剂量,为氯硝柳胺悬浮剂现场灭蚴提供依据。方法杀蚴实验:用25％氯硝柳胺悬浮剂配制氯硝柳胺基质浓度分别为10、5、1、0．5、0．1、0．05、0．01 mg／L溶液,每个浓度组分别取药液0．3 ml置于48孔培养板中,后加入活尾蚴20-50条,解剖镜下观察尾蚴的存活。小鼠感染实验:将200条活尾蚴移入到30 ml的水体中用微型喷雾器均匀喷洒不同浓度氯硝柳胺溶液1 000、100、10、1、0．1、0．01 mg／L于水体表面,喷洒量为药液在水体扩散后整个水体中的终浓度分别为11．6、1．16、0．116、 1．16×10-2、1．16×10-3、1．16×10-4mg／L。喷药后10 min采用浸尾法感染小鼠30 min,小鼠感染 35 d后解剖,观察鼠的虫负荷。结果尾蚴在浓度为10 mg／L和5 mg／L溶液中1 min、1 mg／L和 0．5 mg／L中2 min及0．1 mg／L溶液中30 min死亡率均为100％;在0．05 mg／L和0．01 mg／L溶液中60 min,未见尾蚴死亡。小鼠感染实验显示,经1 000、100、10、1、0．1 mg／L溶液作水面喷洒后,小鼠感染虫负荷显著低于对照组。结论氯硝柳胺杀蚴的有效浓度为0．1 mg／L,随有效浓度的降低,其杀蚴效果逐渐减弱,<0．1 mg／L时,对尾蚴无杀灭作用;用浓度>0．1 mg／L氯硝柳胺悬浮剂对水体表面进行喷洒可起到水体表面灭蚴的作用。
Objective To investigate the niclosamide against the cercariae of Schistosoma killing effect of 25% suspension concentrate of japonicum and ascertain the effect dosage. Methods Experiment against cecariae ： The solutions of niclosamide in the concentrations of 10, 5, 1, 0.5, 0. 1, 0. 05 mg/L and 0.01 mg/L were made of 25% suspension concentrate of niclosamide. The 0.3 ml solution for each concentration group was transfused into a hole of the 48-hole culture plate, 20 --50 cercariae were put into the solution, and the survival situation of cercariae was observed under a dissecting microscope. Experiment infection of mice ： The 200 living cercariae were transfused into 30 ml water in the plastic cup, then the niclosamide solutions with the different concentrations 1 000,100,10,1,0.1 mg/L and 0.01 mg/L were equably sprinkled to the water surface by using subminiature atomizer. The actual concentration of niclosamide after diffusing into water were 11.6, 1.16, 0.116, 1.16×10^-2 ,1.16×10^-3 mg/L and 1.16×10^-4 mg/L. Ten minutes after sprinkling, the tail of mouse was put into each cup for 30 minutes for infection. Thirty-five days after infection, the mice were dissected and worm burden was investigated. Results One hundred percent of cercariae were killed when they were immersed in 10 mg/L and 5 mg/L solutions for 1 minute, or in 1 mg/L and 0.5 mg/L solutions for 2 minutes, or in 0.1 mg/L solution for 30 minutes. No cercaria was killed when they were immersed in 0.05 mg/L and 0.01 mg/L solutions for 60 minutes. The results of infection of mice showed that the worm burdens of mice which were once in 1 000, 100, 10, 1 mg/L and 0.1 mg/L solutions were significantly lower than those in the control solution. Conclusion The effective concentration of niclosamide against schistosome cercariae is no less than 0.1 mg/L. The killing effect against cercariae will weaken with the decreasing concentrations. There is no killing effect against cercariae when the concentration of niclosamide is lower than 0.1 mg/L. There is killing effect against cercariae while using more than 0.1 mg/L solution made of suspension concentrate of niclosamide on water surface by sprinkling.
出处
《中国血吸虫病防治杂志》
CAS
CSCD
2005年第6期424-426,共3页
Chinese Journal of Schistosomiasis Control
基金
江苏省"135工程"医学重点人才基金(ID:RC2002071)卫生部科学研究基金(ID:WKJ2004-2-015)江苏省血吸虫病防治研究所青年科研基金
关键词
25%氯硝柳胺悬浮剂
日本血吸虫
尾蚴
有效浓度
25% suspension concentrate of niclosamide
Schistosoma japonicum
Cercaria
Effective concentration
