摘要
目的探讨载釉基质蛋白(EMPs)的右旋糖酐基凝胶微球(dex_MPs)的制备工艺及其理化与生物学性能。方法采用乳化化学交联技术制备载EMPs的dex_MPs(EMPs_dex_MPs),正交设计法优化制备工艺;观察EMPs_dex_MPs形态和粒径,测定其包封率与载药量;测定微球的溶胀、降解性能,动态透析法检测其体外释药特征及其影响因素;通过EMPs_dex_MPs对体外培养的人牙周膜细胞(PDLCs)增殖和分化的影响,评价其生物学性能。结果EMPs_dex_MPs形态规则,粒径25μm左右,分布较为均匀;EMPs载药量(32.8±1.2)%,包封率(78.9±1.0)%。体外释药实验表明EMPs_dex_MPs中80%的EMPs在前20 d释放,在葡聚糖酶存在下40 d左右可以完全降解。与单纯EMPs相比,EMPs_dex_MPs能持续促进PDLCs的增殖和分化达12 d左右。结论dex_MPs作为活性生长因子载体,具有确定的缓释作用,并可以通过制备工艺的改变控制其释药行为;EMPs_dex_MPs作为EMPs的一种新的给药方式,比传统给药方式可以更加有效地促进牙周组织相关细胞的增殖与分化。
Objective To prepare enamel matrix proteins （EMPs） loaded dextran-based hydrogel microspheres （EMPs-dex- MPs）, and to evaluate their EMPs controlled release property and their biological effects on the proliferation and differentiation of human periodontal ligament cells （PDLCs ） in vitro. Methods Using dimethylbenzene as the oil phase, EMPs-dex-MPs were achieved by emulsion-chemical crosslinking technique. The process of the recombination preparation was optimized by orthogonal factorization method. The configuration and size of EMPs-dex-MPs were determined by scanning electron microscope. The EMPs loading content and encapsulation rate of EMPs-dex-MPs, and their biodegradation characteristic were studied by routine analysis methods. Dynamic dialysis method was used to determine the release characteristic of EMPs-dex-MPs in vitro and its influencing factors. The proliferation of cultured PDLCs was measured by MTT method and the differentiation of PDLCs was measured by their alkaline phosphatase （ALP） activities. Results The results showed that EMPs-dex-MPs were homogenous and stable with the average diameter.25μm, and the EMPs loading content was （32.8± 1.2）%, the encapsulation rate was （78.9 ± 1.0}%. Under 9% physiological saline solution contained a very thimbleful quantity of dextranase EMPs-dex-MPs could be biodegraded completely during about 40 days. The in vitro experiments showed that about 80% of EMPs could be released out in 20 days. Using EMPs- dex-MPs could enhance the proliferation responses and ALP activities of PDLCs mere than 12 days. Conclusion As a new sustained release system of growth factors, the dex-MPs is stable, workable and biodegradable. EMPs-dex-MPs, whose drug release can be controlled by preparation technique, may be more effective in promoting periodontal tissue regeneration.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2005年第6期529-533,共5页
West China Journal of Stomatology
基金
"十五"国家科技攻关计划资助项目(2004BA720A26)
关键词
釉基质蛋白类
右旋糖酐
微球体
控制释放
enamel matrix proteins
dextran
microsphere
controlled release
