摘要
应用TRIzol法分步提取总RNA和总蛋白,提取的总RNA亮度强、完整,提取的总蛋白与经常规RIPA裂解液提取的全蛋白的质量相当。验证了从同一批次处理的细胞中同时提取总RNA和总蛋白进行检测分析是可行的,而且确保了实验条件的一致性,缩短了实验周期。
Total RNA and protein was extracted from the same cell sample by the methods of TRIzol. The results showed that the extracted RNA was integrated enough for the further experiments, and the extracted protein is equal in quality with the protein estracted by the RIPA lysis buffer. So, it is fesible to shorten the period of time and ensure the same experimental conditions.
出处
《生物技术通讯》
CAS
2005年第6期649-650,共2页
Letters in Biotechnology
基金
国家自然科学基金项目(30370348)
北京市自然科学基金项目(7032034)
国家教委博士点基金项目(20010023029)
关键词
TRIZOL法
总RNA
总蛋白
提取
TRIzol method
total RNA extraction
total protein
SDS-PAGE
