摘要
本研究探讨HoxA10基因在急性白血病患者中的表达及其临床意义。运用逆转录聚合酶链反应(RTPCR)方法,检测50例急性白血病患者和10例对照者(7例正常人、3例ITP)中HoxA10mRNA的表达水平;分析其在急性白血病中的表达规律及与临床预后的关系。结果表明:HoxA10在各型急性髓系白血病中均表达,在急性淋巴细胞白血病和部分对照者中低表达。3例正常人不表达HoxA10。HoxA10mRNA表达水平在急性髓系白血病明显高于急性淋巴细胞白血病患者和对照组(P<0.01),在急性髓系白血病中,M1型和M2型表达水平高于M4型和M5型,在M3型有显著高表达。骨髓中原、幼细胞比例和HoxA10mRNA表达水平呈正相关关系(r=0.635,P<0.01)。未缓解组9例患者HoxA10表达水平高于缓解组8例患者,但无显著差异(P=0.258)。1例M2患者和1例M5患者缓解后HoxA10不表达。结论:HoxA10在急性髓系白血病中有异常高表达,具有髓系特异性,可作为区别淋系和髓系的标志性基因,但不是肿瘤细胞特有的基因,而是一类调控造血的转录因子,能与多种协同因子共同影响白血病的发生和发展。HoxA10的表达水平与肿瘤细胞负荷有关,与急性白血病的疗效有一定关系。
To investigate the expression of HoxA10 mRNA in acute leukemia patients and its significanse, HoxA10 level was detected by reverse transcription polymerase chain reaction （RT-PCR） in 50 patients with acute leukemias , 7 healthy volunteers and 3 patients with ITP （ idiopathic thrombocytopenic purpura） . The regularity of the expression of HoxA10 gene in acute leukemia and the relationship between HoxA10 level and the prognosis of leukemia was explored. The results showed that HoxA10 was expressed in all types of acute myelogenous leukemia; HoxA10 message was also observed in acute lymphoblastic leukemia patients and part of control groups. 3 normal donors were found not to express HoxA10. The level of HoxA10 mRNA of acute myelogenous leukemia patients was significantly higher than that of acute lymphoblastic leukemia patients and controls （P 〈 0.01 ）. HoxA10 gene appeared to be more strongly expressed in AML- M1 and -M2 subtypes than in AML-M4 and -M5 subtypes, and the gene was notable high expressed in acute promyelocytic leukemia. The number of blast and promyeloid cells in the bone marrow was positive related with the level of HoxA （r = 0.635, P 〈 0.01 ）. The level of HoxA10 of 9 non-responsive patients was higher than that of 8 remission patients, but there was no significant diffrence between them （ P = 0. 258 ）. HoxA10 was overexpressed in acute myelogenous leukemia. It is concluded that HoxA10 is a major transcription factor regulating hematopoiesis and a mark to differentiate lymphoid leukemia and myelogenous leukemia, but not a specific gene of cancer. The level of HoxA10 is related with load of leukemic cells and curative effect, and can affect occurence and development of leukemia in combination with many cytokines, HoxA10 may facilitate the leukemia progression with another cofactors.
出处
《中国实验血液学杂志》
CAS
CSCD
2005年第6期959-963,共5页
Journal of Experimental Hematology
