摘要
目的了解癌胚抗原信使核糖核酸(CEAmRNA)-癌胚抗原蛋白(CEAp)系统在良、恶性腹水中表达的差别,评估检测CEAmRNA-CEAp系统指标诊断与鉴别诊断良、恶性腹水的应用价值.方法恶性腹水组60例,良性腹水组33例.采集腹水为检测标本,应用反转录套式聚合酶链反应(RT-NP-PCR)技术检测CEAmRNA,磁性抗体分离酶免疫测定(MA-IA)技术检测CEAp.结果良、恶性组腹水中CEAmRNA和CEAp的阳性率分别为3.0%(1/33)和9.1%(3/33),81.7%(49/60)和56.7%(34/60),良、恶性腹水间均具有显著的统计学差异(P<0.01).用CEAmRNA和CEAp指标诊断与鉴别诊断恶性腹水的特异度、敏感度、阳性似然比、阴性似然比、阳性预测值、阴性预测值、准确度分别为0.970和0.909,0.817和0.567,27.233和6.231,0.189和0.476,0.980和0.919,0.744和0.544,0.871和0.699.结论检测腹水中CEAmRNA和CEAp对良、恶性腹水的诊断与鉴别诊断均具有较高的应用价值,其中以CEAmRNA指标更为重要.
AIM： To understand the different expression of carcinoembryonic antigen mRNA （CEAmRNA）-carcinoembryonic antigen protein （CEAp） system in benign and malignant ascites and to assess the value of CEAmRNA-CEAp for the diagnosis and differential diagnosis of benign and malignant ascites. METHODS： Ninety-three patients were examined, among whom 60 cases were with malignant ascites and 33 cases with benign ascites. The ascites were collected and used for CEAmRNA and CEAp detection. CEAmRNA was detected by reverse transcriptase-nested primers-polymerase chain reaction （RT-NP-PCR） and CEAp was detected by magnetic antibody immunoenzymometric assay （MAIA）. RESULTS： The positive rates of CEAmRNA and CEAp were 49 （81.7%） and 34 （56.7%） in the malignant ascites group and 1 （3. 0%） and 3 （9.1%） in the benign ascites group. The positive rates of both CEAmRNA and CEAp in the ascites of patients with malignant ascites were significantly higher than those of benign patients （P 〈 0.01 ）. In the diagnosis and differential diagnosis of malignant ascites, the specificity, sensitivity, the positive likelihood ratio, the negative likelihood ratio, the positive predictive value, the negative predictive value and the accuracy of CEAmRNA and of CEAp were respectively [0.970 and 0.909], [0.817 and0.567], [27.233 and 6. 231], [0. 189 and 0.476], [0.980 and0.919], [0.744 and 0. 544 ], [0. 871 and 0.699 ]. CONCLUSION ： Both CEAmRNA and CEAp are useful indexes for the diagnosis and differential diagnosis of malignant ascites. The diagnostic efficiency of CEAmRNA is higher than that of CEAp.
出处
《第四军医大学学报》
北大核心
2005年第24期2277-2279,共3页
Journal of the Fourth Military Medical University
关键词
癌胚抗原
RNA
信使
癌胚抗原蛋白
诊断
鉴别
恶性腹水
carcinoembryonic antigen
RNA, messenger
carcinoembryonic antigen protein
diagnosis, differential
ascites, malignant
