摘要
目的研究中药姜黄及姜黄素对微囊藻粗毒素致急性肝损伤的化学预防作用及机制。方法52只昆明种♂小鼠随机分为4组:姜黄组,20g·kg-1体重姜黄煎剂灌胃;姜黄素组,300mg·kg-1体重姜黄素灌胃;藻毒素组和对照组生理盐水灌胃,连续灌胃7d。d8,除对照组外,各组腹腔注射微囊藻粗毒素38.11μg·kg-1(0.6LD50),3h后处死动物,取肝制备肝匀浆,检测肝ALT、AST、LDH、ALP、GST、SOD和MDA水平,比较各组变化情况。结果藻毒素组肝匀浆中ALT、LDH、GST分别为(198.65±14.11)U·L-1、(597.47±124.95)U·L-1和(85.23±27.28)kU·g-1Pro,明显高于正常对照组(P<0.05);姜黄组分别为(178.40±13.46)U·L-1、(422.18±105.53)U·L-1和(55.86±13.39)kU·g-1Pro,姜黄素组分别为(168.41±16.13)U·L-1、(220.60±117.36)U·L-1和(54.22±23.87)kU·g-1Pro明显低于藻毒素组(P<0.05);藻毒素组SOD活性(2.08±0.38)kU·L-1明显低于正常对照组(2.62±0.25)kU·L-1(P<0.01),MDA水平为(1.74±0.30)mmol·g-1Pro,明显高于正常对照组(1.10±0.22)mmol·g-1Pro;(P<0.01);姜黄、姜黄素组肝SOD活性分别为(2.47±0.18)kU·L-1和(2.44±0.21)kU·L-1,肝MDA水平分别为(1.30±0.33)mmol·g-1Pro和(1.19±0.21)mmol·g-1Pro,与藻毒素组比较,差别有统计学意义(P<0.01)。其他指标各组之间无统计学意义。结论中药姜黄、姜黄素可明显降低微囊藻粗毒素染毒小鼠肝ALT、LDH和GST升高水平,明显提高染毒小鼠肝SOD活性,降低肝MDA水平,对藻毒素引起的肝脏过氧化损伤具有明显的保护作用。提示中药姜黄和姜黄素可望用于藻毒素等环境肝毒物的化学预防。
Aim To study the chemopreventive effect of curcuma and curcumin on liver injury induced by microcystins（ MC ）in mice. Methods 52 male Swiss mice were randomly divided into 4 groups. They were group curcuma （ ig of 20 g · kg^- 1 curcuma ） , group curcumin （ ig of 300 mg · kg^- 1 curcumin） , group MC and control （ig of 0. 9% saline）. After treating the mice for 7 days, all the mice except control group received intraperitoneal injection of MC at a dose of 38.11 μg · kg^-1（0.6 LD50）. 3 h later, all the animals were sacrificed then the livers were processed for the estimation of ALT, AST, and MDA levels. Results LDH, ALP, GST, SOD Levels of liver homogenate ALT,LDH and GST in group MC were （198.65 ± 14.11） U · L^-1, （597.47 ±124.95） U ·L^-1 and （85.23± 27.28 ） kU · g^-1 Pro respectively, significantly higher than those of the control, P 〈 0. 05. The levels of ALT, LDH and GST in group curcuma were （178.40±13.46） U·L^-1, （422.18±105.53） U· L^- 1 and （55.86 ± 13.39） kU · g^- 1. Pro and in group curcumin were （168.41 ±16. 13） U · L^-1, （220.60 ±117.36） U · L^-1 and （54.22 ＋23.87） kU · g^-1 Pro respectively, markedly lower than those of group MC, P 〈 0.05. A significant increase in MDA level and decrease in SOD activity were found in group MC. Prophylactic administration of curcuma or curcumin resuited in a significant amelioration in the changes of SOD and MDA levels induced by MC, P 〈0.01. Conclusion Hpatic anti-oxidative abilities can be enhanced by curcuma and curcumin,which might act as chemopreventive agents for protection of liver injury induced by MC.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2005年第12期1517-1519,共3页
Chinese Pharmacological Bulletin
