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哇巴因对大鼠心脏内皮素系统表达的影响 被引量：1

Effects of ouabain on endothelin system in rat heart
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摘要 目的探索哇巴因对大鼠心脏内皮素系统表达的影响。方法雄性SD大鼠随机分为哇巴因组和生理盐水对照组,每周测量收缩压,分别于实验24、、6周放免法测定两组大鼠血浆及心脏组织中内皮素的含量,并用免疫组化法检测大鼠左心室内皮素A、B两种受体的表达。结果大鼠腹腔注射哇巴因4周后收缩压开始上升,6周后哇巴因组大鼠血压与对照组相比有显著性差异。大鼠腹腔注射哇巴因2周后,血浆及心脏组织内皮素水平升高,4周时左心室内皮素A型受体表达增强,而B型受体的表达在整个实验过程中均无显著变化。结论哇巴因可以引起血浆及心脏组织中内皮素质量浓度增加,左心室内皮素A型受体表达增强,这种效应可能是其升高血压及损害靶器官的机制之一。 Objective To investigate the effects of a long-term ouabain treatment on endothelin system of rat heart. Methods Male Sprague-Dawley （SD） rats were treated with ouabain or saline for 6 weeks and systolic blood pressure （SBP） were recorded weekly. At the end of 2 weeks, 4 weeks and 6 weeks, the plasma and left ventricle endothelin contents were measured by radio-immunoassay respectively, and the expression of endothelin type A receptor （ETAR） and type B receptor （ETBR） in left ventricle were measured by immuno-histochemistry. Results After 4 weeks, the mean SBP in ouabain group increased. It was significantly higher than that in control group after 6 weeks （P〈0. 001 ）. The plasma and left ventricle endothelin contents were significantly increased after 2 weeks. The upregulation of ETAR in left ventricle was observed after 4 weeks and compared with control group （P〈0.01）. ETBR expression was not altered after ouabain treatment. Conclusion Ouabain can increase circulating and myocardiol endothelin concentration, and upregulate endothelin type A receptor in rat heart. Endothelin system might be involved in the cardiovascular effects of ouabain.
作者 姜馨 郭宁 吕卓人 艾文婷
机构地区 西安交通大学第一医院老年心内科
出处 《西安交通大学学报（医学版）》 CAS CSCD 北大核心 2005年第6期519-522,共4页 Journal of Xi’an Jiaotong University（Medical Sciences）
关键词 哇巴因 内皮素 高血压 ouabain endothelin hypertension
分类号 R544.1 [医药卫生—心血管疾病]
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参考文献10

  • 1任延平,吕卓人.哇巴因与高血压心血管重塑[J].中华高血压杂志,2003,13(z1):35-37. 被引量：2
  • 2张峰,李彬,李玉东.缬沙坦对原发性高血压左室肥厚患者血浆内皮素的影响[J].郑州大学学报（医学版）,2004,39(3):478-480. 被引量：2
  • 3葛衡 吕卓人 于汶.内源性哇巴因在高血压发病学中作用的探讨 [J].中华心血管病杂志,1999,27(4):271-271.
  • 4Barton M,Kiowski W.The therapeutic potential of endothelin receptor antagonists in cardiovascular disease[J].Curr Hypertens Rep,2001,3(4):322-330.
  • 5Giannessi D,Del RS,Vitale RL.The role of endothelins and their receptors in heart failure[J].Pharmacol Res,2001,43(2):111-126.
  • 6Park JB,Schiffrin EL.ET (A) receptor antagonist prevents blood pressure elevation and vascular remodeling in aldosteroneinfused rats[J].Hypertension,2001,37(6):1444-1449.
  • 7Saunders R,Scheiner-Bobis G.Ouabain stimulates endothelin release and expression in human endothelial cells without inhibiting the sodium pump[J].Eur J Biochem,2004,271(5):1054-1062.
  • 8Xavier FE,Yogi A,Callera GE,et al.Contribution of the endothelin and renin-angiotensin systems to the vascular changes in rats chronically treated with ouabain[J].Br J Pharmacol,2004,143(6):794-802.
  • 9Di Filippo C,Filippelli A,Rinaldi B,et al.Chronic peripheral ouabain treatment affects the brain endothelin system of rats[J].J Hypertens,2003,21(4):747-753.
  • 10周洪,张晨晖,李倩虹,牛大地,张新波,周爱儒,温进坤,汤健.内皮素A型受体(ET_A-R)基因在大鼠心血管系统细胞中的表达[J].科学通报,1994,39(12):1137-1140. 被引量：2

二级参考文献26

  • 1[1]Manunta P, Stella P, Rivera R, et al. Left ventricular mass, stroke volume and ouabain-like factor in essential hypertension[J].Hypertension, 1999,34:450-456.
  • 2[3]Purdy RE, Summary of studies of changes in vascular reactivity caused by natriuretic hormones[J].Clin Exp Hypertens,1998,20(5-6):705-716.
  • 3[4]Brudzikowski AS,Leenen FH. Brain 'ouabain' in the median preoptic nucleus mediates sodium-sensitive hypertension in spontaneously hypertension rats[J].Hypertension,1997,29(2):599-605.
  • 4[5]Wang H, Leenen FH. Brain sodium channels mediate increase in brain "ouabain"and blood pressure in Dahl S rats[J].Hypertension,2002,40(1):96-100.
  • 5[6]Taurin S,Dulin NO,Pchejetski D,et al.c-Fos expression in ouabain-treated vascular smooth muscle cells from rat aorta: evidence for an intracellular-sodium-mediated, calcium-independent mechanism[J]. J Physiol,2002,543(pt3):835-847.
  • 6[7]Pchejetski D, Taurin S,Der Sarkissian S, et al. Inhibition of Na+-K+-ATPase by ouabain triggers epithelial cell death independently of inversion of the [Na+]i/[K+]i ratio[J].Biochem Biophys Res Commun,2003,301(3):735-744.
  • 7[8]Stephen CW,Bruce PH,John M, et al.Novel receptors for ouabain :studies in adrenocortical cell and membranes[J].Hypertension,2002,39(pt2):536-542.
  • 8[9]Pierdomenico SD, Bucci A, Manunta P, et al. Endogenous ouabain and hemodynamic and left ventricular geometric patterns in essential hypertension[J].Am J Hypertens,2001,14:44-50.
  • 9[10]Rubaryi GM. The role of endothelium in cardiovascular homestasis and disease[J].J Cardiovasc Pharmacol,1993,22(suppl 4):s1-s4.
  • 10[11]Komiyama Y, Kimura Y, Nishjmura N, et al. Vasodepressor effects of exercise are accompanied by reduced circulating ouabain like immunoreactivity and normalization of nitric oxide synthesis[J].Clin Exp Hypertens, 1997,19(3):363-372.

共引文献8

同被引文献16

  • 1任延平,吕卓人.一种新的内循激素:内源性哇巴因[J].中国医学科学院学报,2005,27(4):534-538. 被引量：3
  • 2谭松涛,杨双强,杨朝坤.Na^+通道阻滞极化停搏液在离体大鼠心脏保存中的保护作用[J].第一军医大学学报,2005,25(10):1283-1285. 被引量：6
  • 3郭宁,姜馨,吕卓人,艾文婷.哇巴因对血管紧张素Ⅱ及其1型和2型受体mRNA在心肌中表达的影响[J].西安交通大学学报（医学版）,2005,26(6):526-529. 被引量：3
  • 4Ferrandi M,Manunta P,Ferrari P,et al.The endogenous ouabain:molecular basis of its role in hypertension and cardiovascular complications[J].Front Biosci,2005,10:2472-7.
  • 5Liu I,Tian J,Haas M,et al.Ouabain interaction with cardiac NA^+-K^+-ATPase initiates signal cascades independent of changes in intracellular Na^+ and Ca^2+ concentrations[J].J Biol Chem,2000,275(36):27-38.
  • 6Pierdomenico SD,Bucci A,Manunta P,et al.Endogenous ouabain and hemodynamic and left ventricular geometric patterns in essential hypertension[J].Am J Hypertens,2001,14:44-50.
  • 7Dostanic I,Paul RJ,Lorenz JN,et al.The alpha2-isoform of Na-K-ATPase mediates ouabain-induced hypertension in mice and increased vascular contractility in vitro[J].Am J Physiol Heart Circ Physiol,2005,288(2):H477-85.
  • 8Saunders R,Scheiner-Bobis G.Ouabain stimulates endothelin release and expression in human endothelial cells without inhibiting the sodium pump[J].Eur J Biochem,2004,271(5):1054-62.
  • 9Ferrandi M,Molinari I,Barassi P,et al.Organ hypertrophic signaling within caveolae membrane subdomains triggered by ouabain and antagonized by PST 2238[J].J Biol Chem,2004,279 (32):33306-14.
  • 10Taurin S,Seyrantepe V,Orlov SN,et al.Proteome analysis and functional expression identify mortalin as an antiapoptotic gene induced by elevation of[Na^+]i/[K^+]i ratio in cultured vascular smooth muscle cells[J].Circ Res,2002,91 (10):915-22.

引证文献1

二级引证文献2

西安交通大学学报（医学版）
2005年 第6期

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