摘要
目的探讨系统性红斑狼疮(SLE)患者外周血中Th1/Th2偏移现象。方法采用外周血单个核细胞(PBMC)培养和酶联免疫吸附试验(ELISA)检测37例SLE患者和17例健康人外周血单个核细胞(PBMC)培养上清液中IL-4I、L-10I、L-12和干扰素-γ(IFN-γ)水平。结果SLE患者的IL-10水平高于健康对照组(P<0.05);IL-12低于健康对照组(P<0.01);IL-4和IFN-γ与健康对照组相比无统计学意义(P>0.05)。结论SLE患者外周血Th1/Th2细胞平衡失调,IL-10升高,IL-12下降。
ABSTRACT： Objective To investigate the imbalance phenomenon of Th1/Th2 ceils of peripheral blood in patients with systemic lupus erythematousus （SLE）. Methods The culture supernatant levels of IL-4, IL-10, IL- 12, and interferon-γ （IFN-γ ）, that were produced by peripheral blood mononuclear （PBMC） were detected by Sandwich ELISA in 37 SLE and 17 healthy subjects. Results The level of IL-10 [（230.94 ± 309. 14）ng/L] produced by PBMC from the patients of SLE was significantly higher than that of normal controls [（85.58± 91.87） ng/L （P〈0.05）]. Thelevclof IL-12 [（24.40±20.08）ng/L] was significantly lower than that of normal controls [（43.53±25.14）ng/L]; the level IL-4 [（32.82±11.31）ng/L] and IFN- γ[（263.80±255.18）ng/L] were not statistically different between SLE patients and controls IL-4 [（27.95±7.55）ng/L, INF- γ （380.61±299.13）ng/L, P〈0.05]. Conclusion There is imbalance of Th1/Th2 cells in patients with SLE： level of IL-10 was higher while level of IL-12 was lower than that in normal controls.
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2005年第6期602-603,共2页
Journal of Xi’an Jiaotong University（Medical Sciences）
基金
江苏省临床免疫重点实验室基金(No.200319)
