摘要
目的探讨降钙素基因相关肽(CGRP)及其拮抗剂(h-CGRP8-37)对正常和应激情况下大鼠胃内胆汁反流的影响。方法SD大鼠65只,实验分三部分:第一部分大鼠20只随机分为四组:对照组、CGRP低、中和高剂量组各5只,分别腹腔注射生理盐水(1ml)和CGRP(10μg/kg 1ml)、CGRP(30μg/kg 1ml)和CGRP(30μg/kg 1ml),0.5h后处死,取胃液测胆汁酸(TBA)浓度。第二部分大鼠30只随机分为二组:应激组和h-CGRP8-37组各15只。从浸入水中开始取2、4、6h共3个时段,每个时段各5只。取胃液测TBA浓度。第三部分大鼠15只,分为对照组、应激即刻组(从应激开始,4小时应激结束)和应激后2h组各5只,测胃液TBA浓度,并用免疫组化SP法检测胃幽门区CGRP神经元阳性反应产物(CGRP-ir)的平均光密度变化。结果正常大鼠在腹腔注射不同剂量CGRP0.5h后胃内胆汁酸浓度明显增高;经幽门局部给予CGRP拮抗剂能显著降低应激性溃疡大鼠在2、46、h三个时段的胃内胆汁返流;免疫组化显示应激性溃疡大鼠胆汁返流增加时,胃幽门区CGRP免疫反应阳性物质活性降低。结论CGRP能促进胃内胆汁酸反流,CGRP参与了幽门括约肌舒张功能的调控。
Objective To study the effects of calcitonin gene-related peptide （CGRP） and its antagonist of CGRP（h-CGRP8-37）in normal and stress rats on bile reflux in stomach. Methods Different dosages of CGRP were administerd IP to the normal rats for half of an hour before being killed and the stress rats were injected the antagonist of CGRP（h-CGRP8-37） into the pyloric sphincters before stress to observe the change of bile acid of gastric juice. CGRP immunoreactivity （ir） neurons in gastric sphincer of the normal group and the stress group for 4 and 6 hours were studied by immunocylochemical SP method. Results The bile acid in CGRP group increased significantly compared with those of normal group. And, the bile acid of h- CGRP8-37 group was less than those of antagonist control group. The activity of the pyloric local CGRP immunoreactivity decreased in the group which were stressed according to image analysis. Conclusion CGRP can promote bile acid reflux in the stomach. CGRP affect and regulate the relaxation function of the pyloric sphincter.
出处
《胃肠病学和肝病学杂志》
CAS
2005年第6期621-624,共4页
Chinese Journal of Gastroenterology and Hepatology
