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快速延迟整流钾通道调节胃肠道自发性电活动 被引量：1

ERG K^+ channels modulatine the spontaneous electrical activity in the gastrointestinal tract
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摘要 目的探讨快速延迟整流钾通道(ether-a-go-go-relatedgene,erg)在CDl小鼠胃肠道的表达及对胃肠道电活动的影响。方法用逆转录聚合酶链反应(RT-PCR)检测erg mRNA在成年CD1小鼠胃肠道的表达;并用细胞外记录法记录和比较erg通道特异性阻滞剂E-4031干预前后离体结肠慢波电位的变化。结果在CDl小鼠胃肠道不同部位均存在着erg mRNA的表达;E-4031干预后,结肠慢波电位频率减慢(P<0.01),时程延长(P<0.01),叠加在慢波电位上的快波电位数量增加,幅度增大,但慢波电位的幅度变化无统计学差异(P>0.05)。结论erg在CDl小鼠胃肠道不同部位表达,ergK+通道在调节胃肠道平滑肌自发性电节律过程中扮演了重要的角色。 Objective To test the expression and possible role of ether-a-go-go-related gene （erg） protein K^＋ channels in gastrointestinal tract of CD1 mice. Methods The expression of erg mRNA in the gastrointestinal tract of CD1 mice were tested by RT-PCR. Extracellular voltage recordings was used to compare the slow-wave activity of colon in the absence or presence of E-4031 （1μM, 30min）. Results RT- PCR showed the presence of ERG mRNA in the gastrointestinal tract of CD 1 mice. E-4031 markedly increased tissue excitability. In the presence of E-4031 （1μm, 2 min）, slow-wave duration increased, slow-wave frequency decreased and the number and amplitude of action potentials superimposing slow-wave increased,but the amplitude of slow-wave didn＇t change significantly. Conclusion erg K^＋ channels contribute to the membrane potential and play a role in modulating spontaneous electrical activity in the gastrointestinal tract.
作者 汪福群 余保平 徐龙 余英
机构地区 武汉大学人民医院消化内科
出处 《胃肠病学和肝病学杂志》 CAS 2005年第6期631-633,共3页 Chinese Journal of Gastroenterology and Hepatology
关键词 快速延迟整流钾通道 慢波电位 功能性胃肠道疾病 Ether-a-go-go-related gene protein K^＋ channel Slow-wave Functional gastrointestinal disorders
分类号 R573 [医药卫生—消化系统]
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参考文献9

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同被引文献13

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胃肠病学和肝病学杂志
2005年 第6期

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