摘要
目的研究PTX3(pentraxin-3)对脂多糖(LPS)诱导内皮细胞表达组织因子(Tissue fac-tor,TF)的影响及其相关机制。方法胰蛋白酶消化法原代培养人脐静脉内皮细胞(HUVEC),一期凝血法检测不同实验组TF促凝活性,酶联免疫吸附实验测TF抗原和反转录-聚合酶链反应(RT-PCR)测TF mRNA表达。结果与对照组比较,单独加入SAP、CRP和PTX3的M199培养基中HUVEC TF促凝活性、抗原和mRNA表达未见明显改变(P>0.05);当与LPS共同培养6 h,每组HUVEC TF活性和抗原均明显升高(P<0.05)。结论PTX3不影响内皮细胞TF促凝活性、抗原和mRNA表达,但参与LPS诱导的内皮细胞表达TF。
Objective To study the effect and mechanism of PTX3 on LPS - induced TF expression in HUVEC. Methods HHVEC were cultured by a typsin digestion methed. After co -incubation with LPS and other factors, the procoagulant activity of TF was assessed by one - step clotting time assay, the TF antigen was determined by ELASA and TF gene mRNA expression was determined by reverse transcriptase - polymerase chain reaction（ RT - PCR）. Results Compared with control group, the SAP,CRP and the PTX3 without LPS group have no changes in HUVEC TF procoagulant activity, TF antigen and TF mRNA expression（ P 〉 0.05）. However, in LPS group and the groups with LPS, HUVEC TF procoagnlant activity, TF antigen and TF mRNA expression significantly increased（ P 〈 0.05）. Conclusion PqX3 is not important to TF procoagulant activity, TF antigen and TF mRNA in HUVEC, but plays a key role in LPS -induced TF expression in HUVEC.
出处
《中国微循环》
北大核心
2005年第6期393-395,399,i0001,共5页
Journal of Chinese Microcirculation
基金
广东省自然科学基金资助(编号04003529)
关键词
PTX3
内皮细胞
组织因子
脂多糖
PTX3
Endothelial cell
Tissue factor
Lipopolysaccharide
