摘要
目的 在比较Alamar Blue法与MTT法的基础上探讨Alamar Blue法用于测定细胞体外增殖及细胞毒实验的可行性。方法 采用Alamar Blue法及MTT法分别测定人肝癌细胞株SMMC-7721的体外增殖以及肿瘤坏死因子相关凋亡诱导配体、顺铂、羟基喜树碱对该细胞株体外增殖的影响。结果 使用Alamar Blue法，接种人肝癌SMMC-7721细胞数在3．12×10^2-2×10^3／孔范围内时，细胞数与Alamar Blue还原率呈线性相关（r=0．9989），高、中、低三种细胞浓度组内差异分别为1．41％、3．88％、4．87％。使用MTT法接种人肝癌SMMC-7721细胞数在3．12×10^2-2×10^4／孔范围内时，细胞数与OD490-620值呈线性相关（r=0．9981），高、中、低三种细胞浓度组内差异分别为1．44％、4．07％、8．27％。结论 Alamar Blue法是一种简便、敏感、安全、经济的方法，可用于体外测定细胞增殖及化疗或免疫制剂的细胞毒作用。
Objective Study the feasibility to measure the proliferation of cells, or to establish toxicity of different chemical agents using the Alamar Blue assay. Methods Alamar Blue assay has been developed for quantitative evaluation of the proliferation of human hepatocellular carcinoma cells SMMC -7721 and the cytotoxicity of TRAIL, cisplatin, and hydroxycamptothecin. A comparison was made between the Alamar Blue assay and the traditional MTT assay. Results The cell density is linearly related to the Alamar Blue reduction using the Alamar Blue assay when the cell density was 3.12 × 10^2 - 2 × 10^4 per well（ r =0.9989 ）. The RSD of three different cell density were 1.41% （2 × 10^4 cells per well） ,3.88% （5 × 103 cells per well） ,4.87% （1.25 × 10^3 cells per well）. The cell density was linearly related to OD490-620 using the MTT assay when the cell density was 3. 12 × 10^2 - 2 × 10^4 per well （ r = 0. 9981 ）. The RSD of three different cell density were 1.44% （2 × 10^4 cells per well）,4.07% （ 5 × 10^3 cells per well） ,8.27% （ 1.25 × 10^3 cells per well）. Conclusion The Alamar Blue assay is a sensitive, economical, simple and nontoxic one -step procedure to evaluate cell proliferation and cytotoxicity.
出处
《现代肿瘤医学》
CAS
2006年第1期6-8,共3页
Journal of Modern Oncology
