摘要
目的:采用高效液相色谱法测定林可霉素利多卡因凝胶中盐酸利多卡因的含量。方法:采用HypersilC18色谱柱(200mm×4.6mm,5μm),以甲醇0.25mol·L1醋酸钠溶液乙腈(55∶26∶19)为流动相,流速1.0mL·min1,紫外检测波长:230nm。外标法峰面积定量。结果:样品与凝胶基质分离良好,线性范围为59.41～138.63mg·L1,样品溶液在24h内稳定,平均加样回收率为99.7%,RSD为0.19%(n=9)。结论:本法简便、快速,结果准确、可靠,重现性好。
OBJECTIVE To determine the content of lidocaine hydroehloride in lincomycin hydrochloride and lidocaine hydrochloride gel by HPLC. METHODS The column ：Hypersil Cas column（5μm,4. 6 mm×200 mm） ;The mobile phase, methanol- 0. 25 mol·L^-1 NaAe- acetonitrile （55：26：19） ;The flow rate ： 1.0 mL·min^-1;The detection wavelength. 230 nm. External method was used. RESULTS The sample and base were separated well by HPLC. The linear response range was 59. 4-138.63 mg·L^-1 ; the sample solution was stable within 24 h. The average recovery was 99. 7%, RSD was 0. 19%. CONCLUSION This method is simple, rapid , accurate and reliable.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2005年第8期723-724,共2页
Chinese Journal of Hospital Pharmacy
关键词
林可霉素
利多卡因
盐酸利多卡因
凝胶
高效液相色谱法
lincomycin hydrochloride and lidocaine hydrochloride gel
lidocaine hydrochloride
HPLC
