摘要
目的:探讨乙酰葛根素在脑缺血再灌注损伤后对一氧化氮(NO)和内皮素(ET)生成的影响。方法:采用大脑中动脉内栓线阻断法造成大鼠局灶性脑缺血再灌注损伤(假手术组除外)后,随机将实验大鼠分为6组,连续灌胃给药10d:损伤组[生理盐水5ml/(kg·d)];对照组[葛根素50mg/(kg·d)];乙酰葛根素高、中、低剂量组[乙酰葛根素250、50、10mg/(kg·d)];假手术组[生理盐水5ml/(kg·d)]。采用生化法测定脑缺血1h再灌注24h及48h大鼠脑组织匀浆和血清中NO水平,用放射免疫法测定血浆中ET水平,并进行脑组织病理学检查。结果:损伤组大鼠脑缺血1h再灌注24h及48h,脑组织匀浆和血清NO、血浆ET水平均明显高于假手术组(P<0.01);与损伤组相比,高、中、低剂量乙酰葛根素组血浆ET及脑组织匀浆NO水平显著降低(P<0.05),血清NO再灌注24h也明显降低(P<0.01);高、中剂量乙酰葛根素组再灌注48h血清NO显著降低(P<0.01)。结论:乙酰葛根素可减少脑缺血再灌注损伤后期不同时间段NO和ET的生成,提示,乙酰葛根素对局灶性脑缺血灌注损伤具有一定的保护作用。
Objective： To investigate the neuroprotective effect of acetylpuerarin on focal brain ischemia-reperfusion injury in rats. Methods： One hundred and eighty rats were averagely divided into 6 groups, which were administered normal saline [5 mg/（kg·d）], puerarin [50 mg/（kg·d）], different dosage of acetylpuerarin [250,50,10 mg/（kg·d）] and normal saline [5 mg/（kg·d）] respectively. Then the NO and ET levels of brain homogenate, serum and plasma with a model of reversible middle cerebral artery occlusion without craniectomy in rats were measured by biochemistry and radio-immunity method. Results： It was found that the levels of NO and ET were increased significantly in ischemia-reperfusion group compared with sham group. NO and ET levels were decreased in acetylpuerarin-treated groups compared with ischemia-reperfusion group. Conclusion： Acetylpuerarin decreases NO and ET production and it may play a role in amelioration of focal brain ischemia repeffusion injury.
出处
《山东大学学报（医学版）》
CAS
北大核心
2005年第12期1173-1177,共5页
Journal of Shandong University:Health Sciences
关键词
乙酰葛根素
脑缺血
再灌注损伤
一氧化氮
内皮素
大鼠
Acetylpuerarin
Brain ischemia
Reperfusion injury
Nitric oxide
endothelin
Rats, Wistar
