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香叶天竺葵组织培养快速繁殖技术研究 被引量:5

Study on tissue culture and high frequency propagation of Pelargonium graveolens L.Her
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摘要 对香叶天竺葵组织培养快速繁殖技术进行了研究,建立了种苗快速繁殖技术规程.结果:适宜的增殖培养基为MS+6-BA为0.5~1.0 mg/ L,继代周期为25~30 d,繁殖系数5以上;生根培养基为1/ 2MS+IBA 0.2 mg/ L,生根诱导率达100 %;生根培养20 d后,将试管苗移栽到菜园土:河沙=1:1的混合基质中,保湿培养,移栽成活率达98.6 %. The propagation technique of Pelargonium graveolens L.Her in vitro were studied and a method for high frequency propagation was established. The results showed that the appropriate medium for different culture stages were: (i)Differentiation and subculture of rosette buds: MS + 6-BA 0.5 - 1.0 mg/L;on this medium shoot multiplication occurred at a up to 5-fold rate after 25 days; (ii)Rooting: 1/2 MS -IBA 0.2 mg/L. In 20 days for rooting, the shoots could be directly rooted in transplantation medium (soil and sand in equal proportion) with 98.6 % survival.
作者 雷开荣
出处 《西南农业学报》 CSCD 2005年第6期794-796,共3页 Southwest China Journal of Agricultural Sciences
基金 重庆市科技创新能力建设专项
关键词 香叶天竺葵 组织培养 快速繁殖 继代周期 繁殖系数 Pelargonium graveolens L. Her tissue culture high frequency propagation
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