摘要
筛选得到一株乳杆菌Lactobacillussp.,进行发酵生产高浓度L-乳酸的研究。考察了种龄、接种量、温度和不同pH调节剂对乳酸发酵的影响。结果表明:最佳种子培养时间为15 h;最佳接种量为15%;最适培养温度为42℃;与氨水和氢氧化钠相比,碳酸钙更适于作为发酵过程的pH调节剂;以葡萄糖为碳源,添加豆粕水解液和玉米浆作为辅料,2 L罐培养120 h,L-乳酸质量浓度可达202 g/L,糖转化率91.3%,L-乳酸占发酵液中总酸含量98%以上。
Production of L-lactic acid by a Lactobacillus sp. was studied. The effects of seed incubation time, inoculation amount, temperature and different pH regulator on L-lactic acid production was investigated. The results showed that the optimal seed inoculation time, optimal inoculation amount and optimal temperature was 15 h, 15 % and 42℃, respectively. Compared with NH3·H2O and NaOH, CaCO3 was better as pH regulator. Using the medium containing glucose, hydrolysate of bean dregs and corn steep liquor with batch operation in 2 L fermenter, final content of L-lactic acid could reach 202 g/L after 120 h. The conversion of glucose was 91.3%, and L-lactic acid occupied more than 98% of total acid.
出处
《生物加工过程》
CAS
CSCD
2005年第4期74-77,共4页
Chinese Journal of Bioprocess Engineering
关键词
L-乳酸
发酵
乳杆菌
L-lactic acid
fermentation
Lactobacillus sp.
