摘要
目的:研究当归药材的指纹图谱.方法:高效液相色谱法,Hypersil ODS柱(4.6 mm×250 mm,5 μm),Kromasil ODS保护柱(4.6 mm×10mm,5 μm).甲醇-1%醋酸梯度流动相,流速1. 0 mL/min,柱温25℃.以当归对照药材为对照品,色谱峰光谱采集范围:190～400 nm;以阿魏酸为参照物并测定了阿魏酸的含量,检测波长:323nm,流动相:甲醇-1%醋酸(40:60,V/V),流速0.7 mL/min.结果:找出了22个共有峰,其中5号峰为阿魏酸,11个样品与当归对照药材之间的相似度均在90%以上,平均相似度为96.77%.结论:样品处理方法简单,研究所得的当归指纹图谱稳定性、重复性好,可以作为当归极性部分的特征性指纹图谱.
Objective： Study on chromatographic fingerprint of Danggui herbs. Method： High-performance liquid chromatography on a Hypersil ODS column with HOAc（1%）-methanol gradient elution, flow-rate of 1.0 mL/min, column temperature of 25 ℃, standard such as Danggui standard, acquisition wave range was 190-400 nm Determined content of ferulic acid with PAD. Mobile phase： Methanol-1% HOAc （60：40, V/V）, flow-rate of 0.7mL/min, detector wave： 323 nm. Result： 22 common peakslwere pointed out, 5th peak was ferulic acid, their similarity of 11 samples were more than 90.0%. Conclusion： Handle method of samples was simple, stable and repeatable. The chromatographic fingerprint of Danggui samples could be used to aqueous solution section of them characterized chromatographic fingerprint.
出处
《分析测试技术与仪器》
CAS
2005年第4期303-306,共4页
Analysis and Testing Technology and Instruments
基金
甘肃省省长基金资助项目(No.GS015-A45-007
GS024-A43-018)
关键词
当归
指纹图谱
高效液相色谱
danggui
chromatographic fingerprint（CFP）
high-performance liquid chromatography
