摘要
To gain insight into the coordination of gene expression profiles under abscisic acid （ABA） and H2O2 applications, global changes in gene expression in response to ABA and H2O2 in Arabidopsis seedlings were investigated using GeneChip （Santa Clara, CA, USA） arrays. Among over 24 000 genes present in the arrays, 459 tran- scripts were found to be significantly increased, whereas another 221 decreased following H2O2 treatment compared with control. Similar to treatment with H2O2, ABA treatment elevated the transcription of 391 genes and repressed that of 322 genes. One hundred and forty-three upregulated genes and 75 downregulated genes were shared between the two treatments and these genes were mainly involved in metabolism, signal transduction, transcription, defense, and resistance. Only two genes, which encode an APETALA2/dehydra- tion-responsive element binding protein （AP2/DREBP） family transcriptional factor and a late embryogenesis- abundant protein, were downregulated by H202, but upregulated by ABA. These results suggest that, similar to ABA, H202 plays a global role in gene transcription of Arabidopsis seedlings. The transcriptional responses induced by the application of exogenous ABA and H2O2 overlapped substantially. These two treatments regulated most of the downstream genes in a coordinated manner.
To gain insight into the coordination of gene expression profiles under abscisic acid （ABA） and H2O2 applications, global changes in gene expression in response to ABA and H2O2 in Arabidopsis seedlings were investigated using GeneChip （Santa Clara, CA, USA） arrays. Among over 24 000 genes present in the arrays, 459 tran- scripts were found to be significantly increased, whereas another 221 decreased following H2O2 treatment compared with control. Similar to treatment with H2O2, ABA treatment elevated the transcription of 391 genes and repressed that of 322 genes. One hundred and forty-three upregulated genes and 75 downregulated genes were shared between the two treatments and these genes were mainly involved in metabolism, signal transduction, transcription, defense, and resistance. Only two genes, which encode an APETALA2/dehydra- tion-responsive element binding protein （AP2/DREBP） family transcriptional factor and a late embryogenesis- abundant protein, were downregulated by H202, but upregulated by ABA. These results suggest that, similar to ABA, H202 plays a global role in gene transcription of Arabidopsis seedlings. The transcriptional responses induced by the application of exogenous ABA and H2O2 overlapped substantially. These two treatments regulated most of the downstream genes in a coordinated manner.
基金
Supported by the National Natural Science Foundation of China （30370765 and 30428026） and the State Key Basic Research and Development Plan of China （2003CB114305）.
