摘要
[目的]探讨柠檬酸化硫酸钙和Osteose t对周围组织作用及对骨修复活动的影响。[方法]建立兔股骨远端包容性骨缺损模型,用柠檬酸化硫酸钙和Osteose t填充缺损,进行一般观察、大体X线及骨组织学计量分析。[结果]空白对照组仅在缺损区边缘有少量的新生骨组织,中央被骨髓组织填充替代。缺损区填充的CCaS和Osteo-se t逐渐降解吸收,被新生的骨组织替代,新生骨组织随时间逐渐成熟,骨小梁逐渐增粗。3、6、13周时CCaS和Osteoset缺损区内新生骨组织量及骨小梁粗度两者无显著性差异。3周时,缺损区内CCaS和Osteose t部分吸收,材料周围有新生骨组织,周边组织未见淋巴细胞浸润及异物巨细胞反应,材料剩余量CCaS组多于Osteose t组(t=21.2,P=0)。[结论]柠檬酸化硫酸钙和Osteose t性质相似、能够修复骨缺损、具有骨传导作用,材料对周围组织无炎症刺激及异物刺激反应。柠檬酸化硫酸钙的降解速度慢于Osteose t,其降解速度更接近于新骨形成速度,有利于新骨的形成,是一种有前途的骨移植替代材料。
[ Objective] To explore the effects on surrounding tissue and bone regeneration by citrated calcium sulphate （CCaS） and Ostesset . [ Method] Bilateral or lateral femoral condyles were drilled into defects in 18 rabbits, and divided into 3 groups： A, 12 rabbits, left defect filled with CCaS cylinder, right defect with osteoset; B, 3 rabbits, left defect with CCaS, right defect blank; C, 3 rabbits, left defect with osteoset, right defect blank. The rabbits were sacrificed at 3 wks, 6 wks and 13 wks, and defect samples were taken for histological exam, roengenographic observation and bone tissue calculation by image-plus CCD system. [Result] The defects that were not grafted were filled with bone marrow and there was minimal new bone, only at the margen of the defects CCaS and osteoset implanted in the defects were gradually absorbed, and substituted by new bone trabecula, which became more mature and thicker. The area fraction and trabecula thickness of new bone in defects treated with CCaS were not significantly different from defects treated with osteoset after 3, 6, 13 wks. However, the degradation velocity of CCaS was slower than that of osteoset. No inflammatory and foreign body response was observed. [ Conclusion] Just like osteoset, CCaS is more benefitial to new bone formation compared with osteoset , because CCaS has relatively slower degradation velocity.
出处
《中国矫形外科杂志》
CAS
CSCD
北大核心
2006年第1期50-52,共3页
Orthopedic Journal of China
