摘要
目的建立桔贝咳喘宁口服液中盐酸麻黄碱的含量测定方法。方法采用高效液相色谱法,选用DiamonsilC18分析柱(4.6mm×150mm,5μm);流动相为0.08mol·L-1磷酸二氢钾-乙腈(90∶10);检测波长:215nm;流速:0.8ml/min;柱温30℃。结果盐酸麻黄碱在0.512～6.144μg范围内有良好的线性关系,r=0.9999;平均加样回收率为98.90%,RSD为1.02%(n=6)。结论本法简便,快速,可用于桔贝咳喘宁口服液的质量控制。
Aim To develop a method for the determination of ephedrine hydrochloride in JubeiKechuanning Oral by high performance liquid chromatography （HPLC）. Methods HPLC method was used. The column was Diamonsil C18 5μm （4.6mm × 150mm）. The mobile phase was consisted of acetonirile - 0.08mol·L^-1 KH2PO4 （10：90）. The detective wavelength was 215nm. Results The regression equation was Y = 63.489 ＋ 1 290. 101X, r = 0.999 9. The average recovery of the method was 98.90%, RSD was 1.02% .The Linear range of ephedrine hydrochloride was 0.512 - 6. 144μg. Conclusion The method is simple, accurate and reproducible. It can be used to determine the ephedrine hydrochloride in JubeiKechuanning Oral.
出处
《解放军药学学报》
CAS
2005年第6期465-466,共2页
Pharmaceutical Journal of Chinese People's Liberation Army
